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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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标示量值七叶皂苷对照提取物的研制

Preparation of aescinate extractive reference standard at labeled amounts

分类号:
出版年·卷·期(页码):2014,34 (7):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:研究制备定量用七叶皂苷对照提取物国家药品标准物质。 方法:参考中国药典2010年版一部娑罗子以及国家药品标准七叶皂苷钠项下方法,以七叶皂苷A、七叶皂苷B、七叶皂苷C、七叶皂苷D为指标,测定七叶皂苷对照提取物中4个皂苷的标示含量。 结果:通过自行纯化、标定4个单体皂苷量值,测得七叶皂苷B、C、D相对于七叶皂苷A的校正因子;通过外标法量值溯源测得七叶皂苷对照提取物中4个皂苷成分的标示含量,分别为:七叶皂苷A 19.33%、七叶皂苷B 17.21%、七叶皂苷C 25.14%、七叶皂苷D 18.10%。同时采用外标法、校正因子法对于标示量值七叶皂苷对照提取物的定值准确性予以验证。 结论:通过单体对照品溯源赋值,建立了定量用七叶皂苷对照提取物标准物质,可用于娑罗子及七叶皂苷类制剂的质量控制分析。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To study and prepare national standard of aescinate extractive reference substance for quantitative analysis. Methods: According to the analysis methods for Aesculi Semen in Chinese pharmacopoeia 2010 edition and the preparation method for sodium aescinate in national drug standards,using ecsin Ⅰa,escin Ⅰb,isoescin Ⅰa and isoescin Ⅰb as indexes,the labeled amounts of four saponins in the aescinate extractive reference substance were determined. Results: Through purification and calibration of the four saponins on our own,the correction factors of escin Ⅰb,isoescin Ⅰa and isoescin Ⅰb relative to escin Ⅰa were obtained.The labeled contents of the 4 saponins in the aescinate extractive reference substance were then detected by the external standard method,which were ecsin Ⅰa:19.33%,escin Ⅰb:17.21%,isoescin Ⅰa 25.14%,isoescin Ⅰb 18.10%.The accuracy of the assigned values for the aescinate extractive reference substance was verified using the external standard method and the correction factor method. Conclusions: Through the assigned traceability of the four saponins,the aescinate extractive reference substance for quantification is prepared,which can be used for quality control analysis of Aesculi Semen and aescine preparations.

-----参考文献:---------------------------------------------------------------------------------------
[1] ChP 2010.Vol Ⅰ(中国药典2010年版.一部)[S].2010:275
[2] Yoshikawa M,Murakami T,Matsuda H,et al.Bioactive saponins and glycosides.Ⅲ.Horse chestnut.(1): The structure,inhibitory effects on ethanol absorption,and hypoglycemic activity of escins Ⅰa,Ⅰb,Ⅱa,Ⅱb and Ⅲa from the seeds of Aesculus hippocastanum L.[J].Chem Pharm Bull,1996,44(8):1454
[3] Guillaume M,Padioleau F.Veinotonic effect,vascular protection,anti-inflammatory and free radical scavenging properties of horse chestnut extract[J].Arzneimittel-Forschung,1994,44(1):25
[4] Wei F,Ma LY,Jin WT,et al.Antiinflammatory triterpenoid saponins from the seeds of Aesculus chinensis[J].Chem Pharm Bull (Tokyo),2004,52(10):1246
[5] Wei F,Ma LY,Cheng XL,et al.Preparatory high performance liquid chromatography for purification of four isomeric bioactive saponins from the seeds of Aesculus chinensis[J].J Liquid Chromatogr Rel Technol,2005,28(5):763
[6] WEI Feng(魏锋),MA Ling-yun(马玲云),MA Shuang-cheng(马双成),et al.HPLC determination of four saponins in Semen Aesculi(HPLC法测定娑罗子中4种皂苷成分的含量)[J].Chin J Pharm Anal(药物分析杂志),2004,24(4):400
[7] GUO JIE(郭杰),XU WEI(徐嵬),YANG Xiu-wei(杨秀伟).Quantified analysis of triterpenoid saponins in Semen Aesculi(娑罗子中三萜皂苷成分的HPLC定量分析) [J].Chin Tradit Herb Drugs(中草药),2007,38(5):767
[8] ChP 2010.Vol Ⅰ(中国药典2010年版.一部)[S].2010:Appendix(附录)30
[9] ZHOU Yu-xin(周玉新).Traditional Chinese Medicine Fingerprint Technology Research(中药指纹图谱研究技术)[M].Beijing(北京):Chemical Industry Press(化学工业出版社),2002
[10] JJG1006-1994,Technical Norm of Primary Reference Materials(一级标准物质技术规范)[S].1994
[11] ISO Guide 35,2006 Reference Materials-General and Statistical Principles for Certifications[S].3rd ed(第三版).2006
[12] ZHU Jing-jing(朱晶晶),WANG Zhi-min(王智民),KUANG Yan-hui(匡艳辉), et al.A quantitative method using one marker for simultaneous assay of ginsenosides in Panax ginseng and P.notoginseng(一测多评法同步测定人参和三七药材中多种人参皂苷的含量)[J].Acta Pharm Sin(药学学报),2008,43(12):1211
[13] Xie PS,Ma SC,Tu PF,et al.The prospect of application of extractive reference substance of Chinese herbal medicines[J].Chin Med,2013,4(4):125
[14] LU Tu-lin(陆兔林),ZHAI Wei-min(翟为民),CAI Bao-chang(蔡宝昌),et al.Application of reference extracts in quality control of traditional Chinese medicines(对照提取物在中药质量控制中的应用) [J].China J Chin Mater Med(中国中药杂志),2013,38(3):462

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