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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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中药复方莲松异搏停片的定性定量方法研究

Study on qualitative and quantitative methods of Liansong Yiboting tablets

分类号:
出版年·卷·期(页码):2014,34 (7):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立中药莲松异搏停片的定性定量方法。 方法:采用薄层色谱法对炙甘草、丹参、莲子心、延胡索、苦参进行定性鉴别。炙甘草TLC条件:1%氢氧化钠溶液制备的硅胶G薄层板,三氯甲烷-甲醇-水(13:7:2)的下层溶液为展开剂;丹参TLC条件:硅胶GF254薄层板,甲苯-三氯甲烷-乙酸乙酯-甲醇-甲酸(2:3:4:0.5:2)为展开剂;莲子心TLC条件:硅胶G薄层板,三氯甲烷-乙酸乙酯-二乙胺(1:6:1)为展开剂;延胡索TLC条件:硅胶G薄层板,环己烷-乙醚-甲醇(3:5:0.5)为展开剂;苦参TLC条件:硅胶G薄层板上,以三氯甲烷-甲醇-氨水(25:1:1)的下层溶液为展开剂。采用高效液相色谱法对炙甘草中甘草酸进行含量测定。HPLC条件:采用Welch Ultimate XB-C18色谱柱(4.6 mm×250 mm,5 μm),流动相为甲醇-磷酸二氢铵溶液(取磷酸二氢铵1.725 g,加水300 mL溶解,用磷酸调节pH至3.5)(61:39),流速1.0 mL·min-1,检测波长251 nm。 结果:定性鉴别斑点清晰,方法稳定可靠;甘草酸在0.19~4.76 μg范围内呈良好的线性关系(r=0.9999),平均回收率(n=9) 为100.2%。 结论:本制剂质量标准所建立方法专属性好,灵敏度高,可用于莲松异搏停片的质量控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish the qualitative and quantitative methods of Liansong Yiboting tablets. Methods: Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle,Salviae Miltiorrhizae Radix et Rhizoma,Nelumbinis Plumula,Corydalis Rhizoma and Sophorae Flavescentis Radix were identified by TLC.TLC condition of Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle:using silica gel G mixed with sodium carboxymethyl cellulose containing 1% sodium hydroxide as the coating substance and the lower solution of chloroform-methanol-water(13: 7: 2) as the mobile phase;TLC condition of Salviae Miltiorrhizae Radix et Rhizoma:using silica gel GF254 as the coating substance and toluene-chloroform-ethyl acetate-methanol-formic acid(2: 3: 4: 0.5: 2)as the mobile phase;TLC condition of Nelumbinis Plumula:using silica gel G as the coating substance and chloroform-ethyl acetate-diethylamine(1: 6: 1) as the mobile phase;TLC condition of Corydalis Rhizoma:using silica gel G as the coating substance and cyclohexane-ethyl ether-methanol(3: 5: 0.5) as the mobile phase;TLC condition of Sophorae Flavescentis Radix:using silica gel G as the coating substance and the lower solution of chloroform-methanol-ammonia(25: 1: 1) as the mobile phase.HPLC conditions of glycyrrhizic acid:using Welch Ultimate XB-C18(4.6 mm×250 mm,5 μm) column as analytic column and methanol-solution of ammonium dihydrogen phosphate(the solution of ammonium dihydrogen phosphate:accurately weigh 1.725 g of ammonium dihydrogen phosphate,add 300 mL of water to dissolve it,adjusted to pH 3.5 with phosphoric acid) (61: 39) as the mobile phase,with a flow rate of 1.0 mL·m in-1 and UV detection at 251 nm. Results: Identification spots were clear,and this method was stable and reliable.The glycyrrhizic acid showed good linear relationship (r=0.9999) in the range of 0.19-4.76 μg.The average recovery(n=9) was 100.2%. Conclusion: This method has a strong specificity,high sensitivity and can be used for quality control of the Liansong Yiboting tablets.

-----参考文献:---------------------------------------------------------------------------------------

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