关闭
 
读者在线:用户名 密码
首页 期刊简介 投稿须知 期刊目录 专家风采 编委会 特邀顾问 联系我们 移动出版
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5



刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

访问统计
您是第  6 3 4 2 3 4 0 位浏览者
您当前的位置:首页 >> 正文

甘氨胆酸中有关物质检查方法的研究

Analysis of related substances in glycocholic acid

分类号:
出版年·卷·期(页码):2014,34 (7):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立HPLC-ELSD法测定甘氨胆酸的有关物质。 方法:采用Gemini C18色谱柱(4.6 mm×150 mm,5 μm),以乙腈-0.1%甲酸(48:52)为流动相,流速0.5 mL·min-1,ELSD检测,漂移管温度95℃,气体流速2.5 L·min-1,Gain为8。 结果:在选定的条件下,甘氨胆酸与其中的5个杂质胆酸、甘氨胆酸乙酯、甘氨脱氧胆酸、甘氨脱氧胆酸乙酯、胆酸乙酯分离良好,它们的检测限相当的杂质浓度分别为0.008%、0.016%、0.016%、0.016%、0.022%、0.016%;5个杂质的平均回收率分别为100.5%、98.1%、105.7%、100.7%、101.4%;且上述6个物质浓度在3.75~31.25 μg·mL-1范围内峰面积的对数与浓度的对数呈良好线性关系。 结论:经方法学验证,本法简便、专属、准确,可用于甘氨胆酸中有关物质的检查。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC-ELSD method to determine the related substances in glycocholic acid. Methods: The HPLC method was performed on a Gemini C18 column(4.6 mm×150 mm,5 μm) with the mobile phase of acetonitrile-0.1 %formic acid(48: 52).The flow rate was 0.5 mL·min-1and the detector was Alltech ELSD 2000 (temperature of drift tube:95℃;airflow velocity;2.5 L·min-1;Gain:8). Results: Under this specific condition,glycocholic acid and its five impurities(cholic acid,ethyl glycocholate,glycodeoxycholic acid,ethyl glycodeoxycholate,ethyl cholate) were well-separated.The limit of detection of glycocholic acid,cholic acid,ethyl glycocholate,glycodeoxycholic acid,ethyl glycodeoxycholate,ethyl cholate was 0.008%,0.016%,0.016%,0.016%,0.022%,0.016% respectively.The average recoveries of the five impurities were 100.5%,98.1%,105.7%,100.7%,101.4%.Linearity was observed within the range of 3.75-31.25 μg·mL-1(r>0.9990) for all the analytes. Conclusion: The method was simple,specific,accurate and suitable for analyzing the related substances in glycocholic acid.

-----参考文献:---------------------------------------------------------------------------------------
[1] XIE Lin(谢麟),CHANG Qing(长青),The Book of Animal Drug Application and Production(动物药剂的应用与制作科技全书)[M].Chengdu(成都):Sichuan Science and Technology Press(四川科学技术出版社),2002:442
[2] ZHAO Zhen(赵珍),LI Pei-feng(李培峰),LI Hui-feng(李慧峰).Studies on extraction and purification techniques of glycocholeic acid from bovne and sheep bile(牛羊胆汁中甘氨胆酸提取纯化工艺研究)[J].J Inner Mongolia Agric Univ(内蒙古农业大学学报),2006,27(2):9
[3] LI Shao-chun(李少春),LI Pei-feng(李培峰).Determining the glycocholic acid of rats blood serum with SPE-HPLC(固相萃取-反高效液相色谱法测定大鼠血清中的甘氨胆酸)[J].Acta Agric Boreali-Sin(华北农学报),2009,24(1):215
[4] ZHAO Huo-zhong(张豁中),WEN Yu-lin(温玉麟).Active Ingredient of Chemical Components(动物活性成分化学)[M].Tianjin(天津):Tianjin Science and Technology Press(天津科学技术出版社),1994:1569
[5] Pyka A,Dolowy M.Separation of selected bile acid by TLC.1.[J].J Liq Chromatogr Relat Technol,2003,26(7):1095
[6] Xiong RL,Lu WG,Li J,et al.Preparation and characterization of intravenously injectable nimodipine nanosuspension[J].Int J Pharm,2008,350(1-2):338
[7] LIU Yu-fang(刘玉芳),YU Lu-li(俞鲁礼),LIU Zhen-hua(刘振华).TLC-scanning determination of bile acid in grass carp(薄层色谱扫描法测定草鱼胆汁酸的含量)[J].J Shanghai Fishers Univ (上海水产大学学报),1995,4(2):152
[8] ZHANG Sui(张穗),HUANG Yu-sheng(黄宇声),SHI Chao-zhou(石朝周).RP-HPLC determination of eight conjugate bile acids in snake gall of gradient elution method(RP-HPLC梯度法测定8种蛇胆中结合型胆汁酸的含量) [J].Chin J Pharm Anal(药物分析杂志),2006,26(6):826
[9] YUE Zhi-hua(岳志华),SUN Hui-min(孙会敏),TIAN Song-jiu(田颂九).Study on simultaneous quantitative determination of cholesterol,bilirubin and various cholic acids in human gallstones by HPLC-ELSD-UV(HPLC-ELSD-UV法同时测定人体胆结石中胆固醇、胆红素及各种胆酸的含量)[J]. Chin J Pharm Anal(药物分析杂志),2004,24(3):310

欢迎阅读《药物分析杂志》!您是该文第 689位读者!

药物分析杂志 © 2009
地址:北京天坛西里2号 邮政编码:100050; 电子邮件:ywfx@nicpbp.org.cn

本系统由北京博思汇文数字科技有限公司设计开发 技术服务电话:400-921-9838