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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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MEKC-DAD同时测定黄芩中6个黄酮类成分的含量

Simultaneous determination of six flavonoids in Scutellariae Radix by MEKC-DAD

分类号:
出版年·卷·期(页码):2013,33 (9):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立胶束电动毛细管色谱二极管阵列检测法(MEKC-DAD)同时测定不同批次黄芩及其炮制品中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A及芹菜素含量。 方法: 以40 mmol·L-1磷酸氢二钠-15 mmol·L-1 硼砂-40 mmol·L-1十二烷基硫酸钠-15%乙腈-7.5%丙醇(pH 8.4)为电泳介质,未涂渍标准熔融石英毛细管(75 μm×64.5 cm,有效长度56 cm)为分离通道,分离电压为20 kV,检测波长为280 nm,毛细管温度为25 ℃,压力进样为5 kPa×6 s。 结果: 6个黄酮的浓度与峰面积的线性关系良好(r>0.9990) ;加样回收率为96.20%~103.6%。用此法测定了黄芩中6个黄酮类成分的含量,得到满意结果。 结论: 该方法简单、准确,重复性较好,可用于黄芩药材内在质量的评价和控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish a method for simultaneous determination of baicalin, baicalein, wogonoside, wogonin, oroxylin A and apigenin in Scutellariae Radix of different batches and its processed products by micellar electrokinetic chromatography with diode array detection(MEKC-DAD). Methods: The buffer solution (pH 8.4) of 40 mmol·L-1 sodium hydrogen phosphate-15 mmol·L-1 sodium borate-40 mmol·L-1 SDS-15% acetonitrile-7.5% propyl alcohol was used as the electrophoretic medium and the standard uncoated fused silica capillary(75 μm×64.5 cm, 56 cm of effective length) was used as the separation pathway. The separation voltage was 20 kV with a detection wavelength of 280 nm; the column temperature was maintained at 25 ℃, and the sample was injected at 5 kPa, 6 s. Results: The above-mentioned six flavonoids showed good linearity(r>0.9990) in the range of the tested concentration, and the average recoveries of the method were between 96.20%-103.6%. Conclusion: The method is simple, accurate and reproducible, and can be used for quality control and evaluation of Scutellariae Radix.

-----参考文献:---------------------------------------------------------------------------------------
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3 KAN Hong-yu(阚红玉), SONG Dian-rong(宋殿荣), WANG Yue-fei(王跃飞), et al. Determination of 5 kinds of flavonoids in Scutellaria baicalensis by HPLC(HPLC法同时测定黄芩中5种黄酮类成分的含量). China Pharm(中国药房), 2010, 21(11): 1016
4 CHEN Pei-dong(陈佩东), XU Dan-yang(徐丹洋), KONG Xiang-peng(孔祥鹏), et al. Isolation and identification of flavonoids in Scutellaria baicalensis and study on their effects on in vitro coagulation system(黄芩中黄酮类成分的分离鉴定及其体外对凝血系统的影响). Chin Tradit Herb Drugs(中草药), 2012, 43(12): 2333
5 ZHOU Xi-qin(周锡钦), ZHANG Qing-ying(张庆英), LIANG Hong(梁鸿), et al. Analysis of main flavonoids contents of Scutellaria baicalensis(黄芩中主要黄酮类成分的含量分析). China J Chin Mater Med(中国中药杂志), 2009, 34(22): 2910
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9 SHI Cun-yi(时存义), SUN Guo-xiang(孙国祥), SONG Wen-jing(宋文璟). Capillary eletrophoresis fingerprints of Radix Scutellariae and determination of baicalin by CZE(黄芩的毛细管电泳指纹图谱和黄芩苷含量测定研究).Chin Tradit Pat Med(中成药), 2008, 30(4): 469
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11 Zhang H, Tian K, Tang J, et al. Analysis of baicalein, baicalin and wogonin in Scutellariae Radix and its preparation by microemulsion electrokinetic chromatography with 1-butyl-3-methylimizolium tetrafluoborate ionic liquid as additive. J Chromatogr A, 2006, 1129(2): 304
12 QI Li(齐莉), ZHOU Rong(周蓉), WANG Ya-fen(王雅芬), et al. Isolation of effective flavonoids contents of Scutellaria baicalensis by MEKC(用胶束电动毛细管色谱法分离黄芩中黄酮类有效成分). Chem Anal Meter(化学分析计量), 1999, 8(3): 10

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