HPLC-ESI-MS和HPLC法分析测定灵芝醇提物中3种灵芝酸

目的: 对灵芝醇提物中灵芝酸A、B、C₂的含量进行测定,为灵芝醇提物的质量控制提供依据。 方法: 采用高效液相色谱-电喷雾-质谱(HPLC-ESI-MS)法对灵芝醇提物中的灵芝酸A、B、C₂进行定性分析,采用HPLC法对3种灵芝酸的含量进行测定。 结果: HPLC-ESI-MS法测定结果显示,灵芝醇提物含有灵芝酸A、B、C₂。HPLC法测定结果显示,灵芝酸A、B、C₂分别在3.50~525.60 μg · mL ^-1(r=1.0000)、3.23~484.80 μg · mL ^-1(r=1.0000)、2.04~203.6 μg · mL ^-1(r=0.9999)浓度范围内呈现良好的线性。平均回收率分别为98.2%,101.5%,98.7%(n=9)。不同来源的赤芝、紫芝醇提物中灵芝酸A、B、C₂总含量差异较大,灵芝酸A在3种灵芝酸中含量最高,紫芝醇提物中灵芝酸A、B、C₂的总含量较赤芝醇提物的低。 结论: 该方法稳定性、耐用性良好,可用于灵芝醇提物的质量控制。

Objective: To determinate the contents of ganoderic acids A,B,C₂ in Lingzhi ethanol extracts,so as to provide a basis for quality control of Lingzhi ethanol extracts. Methods: The methods of HPLC-ESI-MS and HPLC were adopted for qualitative and quantitive analyses of ganoderic acids A,B,C₂ in Lingzhi ethanol extracts. Results: The HPLC-ESI-MS analysis showed that Lingzhi ethanol extracts contained ganoderic acid A,B,C₂.The HPLC analysis showed that the method had good linearity for ganoderic acid A,B,C₂ in the range of 3.50-525.60 μg·mL ^-1(r=1.0000),3.23-484.80 μg·mL ^-1(r=1.0000),2.04-203.6 μg·mL ^-1(r=0.9999),respectively.The average recoveries of ganoderic acid A,B,C₂ were 98.2%,101.5%,98.7% (n=9),respectively.The contents of total ganoderic acids were different in the same species and the ganoderic acid A was the major compound of the three ganoderic acids.The total contents in G.sinensis ethanol extracts were far lower than those in the G.lucidum. Conclusion: The methods are stable and durability,which can be used for the quality control of Lingzhi ethanol extracts.