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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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续断高效毛细管电泳指纹图谱研究

Study on high performance capillary electrophoresis fingerprint of Dipsaci Radix

分类号:
出版年·卷·期(页码):2012,32 (7):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 利用高效毛细管电泳(HPCE)方法建立中药续断的指纹图谱。 方法: 采用50 μm×65 cm(有效长度57 cm)未涂层石英毛细管柱,以pH 9.20,50 mmol·L的硼砂-硼酸为缓冲溶液体系,运行电压25 kV, 温度25℃,压力进样5 kPa×30 s, 检测波长212 nm。采用"中药色谱指纹图谱相似度评价系统2004A版"软件和聚类分析方法,对样品图谱进行分析。 结果: 12批续断HPCE指纹图谱共确认了14个共有指纹峰,其中产自湖北的2批药材相似度介于0.8至0.9之间,产自四川的10批药材相似度大于0.9。聚类与相似度结果一致。 结论: 此法准确、可靠,可为续断药材的质量评价与控制提供科学依据。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To investigate the HPCE fingerprint of Dipsaci Radix. Methods: The HPCE fingerprint was established using a 50 μm× 65 cm uncoated fused silica capillary(effective length 57 cm),a buffer comprising 50 mmol·L-1 borate-boric acid at pH 9.20,a running voltage of 25 kV,a capillary temperature of 25℃ and a wavelength of 212 nm.The sample was injected under pressure of 5 kPa for 30 s.The HPCE fingerprint was evaluated by the software "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine Version 2004A" and clustering analysis. Results: By analyzing the HPCE fingerprint,high similarities were found among the 12 samples.The similarity coefficients of the two batches of samples from Hubei were from 0.8 to 0.9,and those of the remaining samples from Sichuan were greater than 0.9.Fourteen major co-possessing peaks were selected as the characteristic fingerprint peaks of Dipsaci Radix. Clustering results were consistent with the similarity analysis. Conclusion: The developed method is accurate and reliable,and the fingerprint analysis can thus be used for the quality assessment and control of Dipsaci Radix.

-----参考文献:---------------------------------------------------------------------------------------
1 ChP(中国药典).2010.VolⅠ(一部): 309
2 GAO Xiu-zhi(高秀芝),MA Lu-yu(马鲁豫),JIN Yan-xia(金艳霞),et al.Advances of studies on chemical constituents and pharmacological actions of Dipsacus asperoides(川续断化学成分及药理作用研究进展).Asia-Pacific Tradit Med(亚太传统医药),2010,6(7): 143
3 LUO Guo-an(罗国安),FU Chan(付婵),LI Sha-sha(李莎莎),et al.Study on HPLC fingerprint chromatography of Zhenwu decoction(真武汤的HPLC指纹图谱研究).Chin J Pharm Anal(药物分析杂志),2011,31(5): 892
4 WEI Ying-jie(韦英杰),JIA Xia-bin(贾晓斌),FAN Hong-wei(樊宏伟),et al.Chromatographic fingerprint of Dipsacus asper and analysis by HPLC-MS(续断的色谱指纹及LC-ESI-MS分析).China J Chin Mater Med (中国中药杂志),2011,36(2): 169
5 LIU Yong(刘永),WEI Ying-fang(卫莹芳),ZHU Jun-ping(朱俊平),et al. Fingerprint analysis of Dipsacus asperoides by HPLC(续断HPLC指纹图谱研究).J Chin Med Mater(中药材),2010,33(3): 359
6 LIU Feng-le(刘凤乐),YANG Zhong-lin(杨中林),LIU Yun-fei(刘云飞),et al. Study on HPLC fingerprint of Dipsacus asperoides(续断药材的HPLC指纹图谱研究). J Chin Med Pharmacol(中医药学报),2009,37(5): 62
7 YAN Yong-xin(晏永新),ZHONG Tie(钟铁),ZHANG Xiao-juan(张小娟), et al.HPLC fingerprint of processing Flos Lonicerae from different habitats(山银花不同产地加工品的HPLC指纹图谱研究).Chin Tradit Herb Drugs(中草药),2007,38(11): 1643
8 SUN Guo-xiang(孙国祥),LIU Xiao-ling(刘晓玲),DENG Xiang-yu(邓湘昱),et al.The index F and relative index Fr of chromatographic fingerprints(色谱指纹图谱指数F和相对指数Fr的研究).Acta Pharm Sin(药学学报),2004,39(11): 921

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