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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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五加生化胶囊UPLC-MS指纹图谱研究

UPLC-MS fingerprint of Wujia Shenghua capsules

分类号:
出版年·卷·期(页码):2012,32 (7):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立五加生化胶囊UPLC-MS的指纹图谱,为科学客观地评价其质量提供可靠的方法。 方法: 色谱柱:ACQUITY UPLC BEH (2.1mm×50 mm,1.7 μm);流动相:乙腈(A)-0.1%甲酸和10 mmol·L-1醋酸铵(B)梯度洗脱;柱温:40℃;流速:0.4 mL ·min-1;进样量:8 μL;电喷雾接口,正离子模式,毛细管电压3.50 kV,锥孔电压35.00 V,离子源温度120℃,脱溶剂温度380℃,脱溶剂气N2:500 L·h-1,锥孔气N2:50 L ·h-1结果: 11批五加生化胶囊UPLC-MS指纹图谱中共标定了37个共有色谱峰,通过与对照品的保留时间及质谱信息的比对,确定4号、8号、9号、10号和30号峰分别为紫丁香苷、阿魏酸、甘草苷、异嗪皮啶和藁本内酯。 结论: 所建立的五加生化胶囊UPLC-MS指纹图谱满足于方法学的要求,所得的指纹图谱特征性及专属性强,可用于五加生化胶囊质量的控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To develop a ultra-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (UPLC-ESI-MS) method for chemical fingerprint analysis of Wujia Shenghua capsules. Method: A UPLC-MS system, an ACQUITY Waters Ultra-Performance Liquid Chromatographic coupled with a Micromass Quattro Premier XE electrospray ionization (ESI) source mass spectrometer was used. An ACQUITY UPLC BEH (2.1mm×50 mm, 1.7 μm) column was operated under a gradient of 10 mmol·L-1 ammonium acetate and 0.1% formic acid in water. The parameters of mass spectrometer were optimized. The spectra were recorded in the m/z range of 100-1 000. Result: Among 11 batches Wujia Shenghua Capsules, 37 common peaks were defined and identified by UPLC-MS. By comparing MS spectra data with the authentic standards and literature, the original of common peaks were determined and peak 4, 8, 9, 10 and 30 were identified as syringin, ferulaic acid, liquirtin,isofmxidin and ligustilide, respectively. Conclusion: The developed fingerprint assay is specific and can be readily utilized for comprehensive evaluation and quality control of Wujia Shenghua capsules.

-----参考文献:---------------------------------------------------------------------------------------
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4 ZHANG Feng (张凤), DONG Yu-guo (董玉国). Pharmacological effects and clinical application of Wujia Shenghua Capsule(五加生化胶囊药理作用及临床应用). Mod Sci (今日科苑),2009, (8): 290
5 ZENG Qiao-yun (曾巧云). The clinical observation of prevention of Analgesia stream of people complications (预防无痛人流术后并发症的临床察). Heilongjiang Med Pharm (黑龙江医药科学), 2008(5):55
6 LUO Guo-an(罗国安), FU Chan(付婵), LI Sha-sha(李莎莎), et al. Study on HPLC fingerprint chromatography of Zhenwu decoction (真武汤的HPLC指纹图谱研究). Chin J Pharm Anal (药物分析杂志),2011,31(5):892
7 SUN Guo-xiang(孙国祥), YU Xiu-ming(于秀明), WANG Jia-qing(王佳庆).Study on the digitized HPLC fingerprints of Ciwujia injection (刺五加注射液HPLC数字化指纹图谱研究). Chin J Pharm Anal (药物分析杂志),2009,29(3):356

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