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期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC法测定脂肪乳注射液及卵磷脂中溶血磷脂的含量

HPLC determination of lysophosphatidyl choline in fat emulsion injection and lecithin

分类号:
出版年·卷·期(页码):2012,32 (6):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立高效液相色谱法测定脂肪乳注射液及卵磷脂中溶血磷脂。 方法: 采用Alltima Silica色谱柱,以甲醇-冰醋酸(100∶0.2,三乙胺调节pH至6.0)为流动相,使用蒸发光散射检测器,漂移管温度:70 ℃,气体:空气,流量:1.8 L·min-1结果: 在选定的条件下,溶血磷脂与样品中其他组分分离良好。溶血磷脂的检测限为0.1 μg;溶血磷脂在0.04~0.20 mg·mL-1范围内,峰面积的对数与浓度的对数呈良好的线性关系;平均回收率为103.0%,RSD为1.8%。 结论: 本法简便、专属、准确,可用于脂肪乳注射液及卵磷脂中溶血磷脂的含量测定。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To develop an HPLC method for determination of lysophosphatidyl choline in fat emulsion injection and lecithin. Method: The HPLC method was performed on an Alltima Silica column with methanol-acetic acid(100∶0.2,adjusted pH 6.0 with triethylamine) as mobile phase,and the detector was ELSD,of which the drift tube temperature was maintained at 70 ℃ and the flow rate of carrier gas was kept at 1.8 L·min-1. Result: Under the described condition,lysophosphatidyl choline was completely separated from other components in fat emulsion injection and lecithin.The LOD of lysophosphatidyl choline was 0.1 μg.The linear range of lysophosphatidyl choline was 0.04-0.20 mg·mL-1,and the average recovery was 103.0%,RSD=1.8%(n=9). Conclusion: The method is simple,specific and accurate,and can be used for determination of lysophosphatidyl choline in fat emulsion injection and lecithin.

-----参考文献:---------------------------------------------------------------------------------------
1 HUANG Yuan(黄媛),YANG Liu(杨柳),CHAO Ruo-bing(晁若冰),et al.TLC building of lysophosphatidyl choline in fat emulsion injection and phospholipids(薄层色谱法检查脂肪乳注射液及卵磷脂中的溶血磷脂酰胆碱).Chin J Pharm Anal(药物分析杂志),2010,30(12):2334
2 LING Li-xin(凌立新),YANG Shi-jun(杨仕军),LANG Tao(郎涛).The research of determination of lysophosphatidyl choline in Soybean Phospholipid(大豆磷脂中溶血磷脂酰胆碱含量测定方法研究).Asia-Pacific Tradl Med(亚太传统医药),2009,5(1):35
3 WU Hui-ai(吴惠爱),MAI Xiao-hong(麦小虹),HU Rong-rong(胡容融).Determination of major components in ovolecithin for injection by HPLC-ELSD(HPLC-ELSD检测系统测定注射用卵磷脂主要组分的含量).Acad J Guangdong Coll Pharm(广东药学院学报),2003,19(3):228
4 YUAN Xin-rong(袁昕蓉),QIU Zhi-bin(邱志斌),WANG Dong-kai(王东凯).Content determination of 5 constituents in lecithin by HPLC-ELSD(HPLC-ELSD法测定卵磷脂中5种主成分的含量).China Pharm(中国药房),2009,20(31):2465
5 CHEN Miao-fen(陈妙芬),WANG Dan-jing(万丹晶),CHEN Gang(陈刚),et al.Determination of the concentrations of 6 components in lecithin by HPLC method(HPLC法测定卵磷脂中6个成分的含量).Pharm Care Res(药学服务与研究),2005,5(3):234
6 WAN Yi-qun(万益群),WU Shi-fang(吴世芳).Analysis of phosphatides in Polygonum multiflorum by HPLC-ELSD(高效液相色谱-蒸发光散射检测法分析何首乌中磷脂类化合物).J Anal Sci(分析科学学报),2008,24(4):417

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