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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC测定丹参素冰片酯脂肪乳注射液含量及有关物质

HPLC determination of content and related substances of Danshensu Borneol Ester fat emulsion injection

分类号:
出版年·卷·期(页码):2012,32 (5):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立丹参素冰片酯脂肪乳注射液含量测定及有关物质检查方法。 方法: 采用高效液相色谱法。色谱柱:Kromasil C18(150 mm×4.6 mm,5 μm)色谱柱,流动相:乙腈-0.2%甲酸水(42:58),流速为1.0 mL·min-1,检测波长280 nm,进样体积20 μL。 结果: 试验结果表明,HPLC法检测主峰与杂质峰有较好的分离度,且重复性较好;丹参素冰片酯的浓度在5~60 μg·mL-1范围内与峰面积呈良好线性关系(r=1.000),平均回收率为98.2%(n=9)。 结论: 该法操作简便,结果可靠,适用于丹参素冰片酯脂肪乳注射液含量测定及有关物质检查。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish a method to determine the content and related substances of Danshensu Borneol Ester fat emulsion injection. Methods: The experimental conditions were as follows: Kromasil C18 column(150 mm×4.6 mm, 5 μm), acetonitrile-0.2% formic acid in water(42:58) as mobile phase,the flow rate: 1.0 mL·min-1 ,the injection volume: 20 μL, and detection wavelength was set at 280 nm. Results: The main peak and impurity peaks have good resolution and repeatability. The calibration curve was linear in the range of 5-60 μg·mL-1(r=1.000). The average recovery was 98.2%(n=9). Conclusion: The method is simple, rapid, sensitive and accurate. It is suitable for determination of the content and related substances of Danshensu Borneol Ester fat emulsion injection.

-----参考文献:---------------------------------------------------------------------------------------
1 LIU Shao-jing (刘少静).Study on structural characterization, quality control and pharmacokinetic of DBZ(DBZ的结构表征、质量控制及其药代动力学研究):.Shaanxi(陕西):Northwestern University(西北大学),2009
2 ZHENG Xiao-hui(郑晓晖),ZHANG Qun-zheng(张群正),WANG Shi-xiang(王世祥),et al.β-(3,4-dihydroxyphenyl)-α-hydroxy acid borneol esters,synthesis and use(β-(3,4-二羟基苯基)-α-羟基丙酸冰片酯、其合成方法和用途).CN:200610042787,2006-11-29
3 ChP(中国药典).2005.Vol Ⅱ(二部):Appendix(附录)172
4 State Food and Drug Administration(国家食品药品监督管理局).Technical Guidelines for Drug Research(药物研究技术指导原则).Beijing(北京):China Medico-Pharmaceutical Science and Technology Publishing House(中国医药科技出版社),2005.211,50
5 SUN Hao(孙浩),JIANG Su-mei(蒋素梅),GU Ping(顾萍),et al.Determination of repaglinide and the related substances in repaglinide and its tablets by HPLC(HPLC法测定瑞格列奈原料药及其片剂中主药和有关物质的含量).China Pharm (中国药房),2007,18(19):1496
6 YAO Jing(姚静),ZHANG Qi-ming(张启明),LI Zhuo-rong(李卓荣),et al.HPLC determination of repaglinide tablets(高效液相色谱法测定瑞格列奈片的含量).Chin J Pharm Anal(药物分析杂志),2007,27(5):728
7 Liu D,Gao YW,Wang HJ,et al.Evaluation of the effects of cytochrome P450 nonsynonymous single-nucleotide polymorphisms on Tanshinol Borneol Ester metabolism and inhibition potential.Drug Metab Dispos,2010,38(12):2259
8 LIU Shao-jing(刘少静),LI Xia(李霞),NAN Ye-fei(南叶飞),et al.Quality control of isopropyl β-(3,4-dihydroxyphenyl)-α-hydroxypropanoate by HPLC(HPLC法控制丹参素异丙酯质量).J China Pharm Univ(中国药科大学学报),2009,40(1):47

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