关闭
 
读者在线:用户名 密码
首页 期刊简介 投稿须知 期刊目录 专家风采 编委会 特邀顾问 联系我们 移动出版
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5



刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

访问统计
您是第  7 3 1 6 6 2 3 位浏览者
您当前的位置:首页 >> 正文

HPLC测定11种中药挥发油中桉油精的含量

Determination of eucalyptol in 11 kinds of herbal volatile oil by HPLC

分类号:
出版年·卷·期(页码):2012,32 (5):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立中药挥发油中桉油精含量的HPLC检测方法。 方法: 采用Dikma Kromasil C18(250 mm×4.6 mm,5 μm)色谱柱,流动相为乙腈-水(64: 36),流速1.0 mL·min-1,检测波长203 nm,柱温30 ℃。 结果: 桉油精进样量在4.72~47.2 μg范围内具有良好的线性关系(r=0.9996);平均回收率为99.3%,RSD为0.86%。 结论: 该方法简便快速、准确,具有良好的重复性和回收率,可作为中药挥发油中桉油精的定量分析方法。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC method for the content determination of eucalyptol. Methods: The separation was performed on a Dikma Kromasil C18(250 mm×4.6 mm,5 μm)column.The mobile phase was acetonitrile-water(64: 36)at the flow rate of 1.0 mL·min-1.The detective wavelength was set at 203 nm,and the column temperature was 30 ℃. Results: The calibration curve was linear in the range of 4.72-47.2 μg(r=0.9996).The average recovery was 99.3% with RSD of 0.86%. Conclusion: This method is simple,rapid,accurate and with good repeatability and recovery,and can be used as a quantitative analysis method for eucalyptol in herbal volatile oil.

-----参考文献:---------------------------------------------------------------------------------------
1 ChP(中国药典).2010.Vol Ⅰ(一部):Appendix(附录)Ⅹ C
2 CAI Zhao-nan(蔡照南),LI Xue-yu(李雪玉),XU Xing-wen(徐兴文).GC determination of eucalyptol in Eucalyptus oil(气相色谱法测定桉油中桉油精的含量).China J Chin Mater Med(中国中药杂志),1990,15(5):42
3 WANG Gang-li(王钢力),GAO Tian-bing(高天兵),LIN Rui-chao(林瑞超),et al.GC determination of eucalyptol in Rhizoma Alpiniae Officinarum(气相色谱法测定高良姜中桉油精含量).Drug Stand China(中国药品标准),2003,4(2):37
4 WANG Yu-lan(王玉兰),YU Li-ming(于黎明),REN Wei(任玮),et al.GC determination of eucalyptol in essential oil of Fructus Tsaoko(气相色谱法测定草果挥发油中桉油精的含量).Lishizhen Med Mater Med Res(时珍国医国药),2004,15(12):822
5 SUN Ye(孙晔),CHEN Xi(陈希),ZHANG Hui(张蕙),et al.GC determination of eucalyptol,Artemisia ketone,camphor,caryophyllene and caryophyllene oxide in Artemisia annua L.produced in Sichuan(川产黄花蒿中桉油精、蒿酮、樟脑、石竹烯、氧化石竹烯含量的气相色谱法测定).Chin J Pharm Anal(药物分析杂志),2006,26(2):239
6 WANG Xing-ling(王兴宁),ZHANG Ming-shi(张明时),XIA Pin-hua(夏品华),et al.Determination of eucalyptol in Flos Magnoliae by capillary GC(毛细管气相色谱法测定辛夷药材中桉油精含量).Lishizhen Med Mater Med Res(时珍国医国药),2008,19(1):48
7 JIANG Ping-chuan(姜平川),LI Jia(李嘉),CHEN Feng(陈锋),et al.Identification and determination of linalool and eucalyptol in compound Qianhuang gel(复方千黄凝胶的鉴别及芳樟醇和桉油精的含量测定).Chin J Pharm Anal(药物分析杂志),2010,30(6):987
8 ChP(中国药典).2010.Vol Ⅰ(一部):388
9 LIN Yan-zhi(林燕芝),TANG Xing(唐星),BI Kai-shun(毕开顺).Determination of ligustilide in volatile oil from rhizome of Ligusticum chuanxiong by RP-HPLC(RP-HPLC测定川芎挥发油中藁本内酯的含量).Lishizhen Med Mater Med Res(中国中药杂志),2004,29(2):154
10 JIA Tian-zhu(贾天柱),SHA Ming(沙明),WANG Zhong-hai(王忠海),et al.Determination of myristicin and safrol in volatile oil of Nutemeg and its processed products by HPLC(肉豆蔻不同炮制品挥发油中肉豆蔻醚、黄樟醚的HPLC法测定).China J Chin Mater Med(中国中药杂志),1997,22(7):410
11 YAN Ke-li(阎克里),ZHU Xiu-qing(朱秀卿),ZHAO Li(赵丽).HPLC determination of atractylon,β-eudesmol and β-elemene in volatile oil of Atractylodes macrocephala Koidz.(高效液相色谱法测定白术挥发油中苍术酮、β-桉叶醇、榄香烯的含量).Chin Rem Clin(中国药物与临床),1997,22(7):410
12 HAN Jing(韩静),TANG Xing(唐星),BA De-chun(巴德纯).Determination of nerolidol in volatile oil from Lignum Dalbergiae Odoriferae by HPLC(HPLC法测定降香挥发油中橙花叔醇的含量).Chin Tradit Herb Drugs(中草药),2004,35(7):824

欢迎阅读《药物分析杂志》!您是该文第 1523位读者!

药物分析杂志 © 2009
地址:北京天坛西里2号 邮政编码:100050; 电子邮件:ywfx@nicpbp.org.cn

本系统由北京博思汇文数字科技有限公司设计开发 技术服务电话:010-60213898