关闭
 
读者在线:用户名 密码
首页 期刊简介 投稿须知 期刊目录 专家风采 编委会 特邀顾问 联系我们 移动出版
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5



刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

访问统计
您是第  8 0 1 6 4 8 5 位浏览者
您当前的位置:首页 >> 正文

HPLC同时测定山核桃外蒲壳中球松素和大黄酚含量

HPLC simultaneous determination of pinostrobin and chrysophanol in hull of Carya cathayensis Sarg.

分类号:
出版年·卷·期(页码):2012,32 (2):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立同时测定山核桃外蒲壳中球松素和大黄酚含量的分析方法。 方法: 采用HPLC法,色谱柱为Shim-pack C18(250 mm×4.6 mm,5 μm),以甲醇-0.1%磷酸水溶液(79∶21)为流动相,流速0.8 mL·min-1,检测波长286 nm,柱温25 ℃。 结果: 山核桃外蒲壳中球松素和大黄酚的线性范围分别为4.12~25.7 μg·mL-1和1.01~13.4 μg·mL-1,平均加样回收率(n=6)分别为98.8%和98.0%。 结论: 该方法准确可靠、结果稳定、重复性好,为评价山核桃外蒲壳的质量提供依据。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish a method for simultaneous determination of pinostrobin and chrysophanol in the hull of Carya cathayensis. Methods: The analysis was carried out on a Shim-pack C18(250 mm×4.6 mm,5 μm) column eluted with a mobile phase of methanol-water containing 0.1% phosphoric acid(79∶21);The flow rate was 0.8 mL·min-1 and column temperature was 25 ℃;The detection wavelength was set at 286 nm. Results: The calibration curves of pinostrobin and chrysophanol were linear in the ranges of 4.12-25.7 μg·mL-1 and 1.01-13.4 μg·mL-1,and the average recoveries(n=6) were 97.8% and 98.0%,respectively. Conclusion: The method is simple,accurate and sensitive,which can be used as a method of quantitative determination of pinostrobin and chrysophanol in the hull of Carya cathayensis.

-----参考文献:---------------------------------------------------------------------------------------
[1] CHEN Shi-yun(陈仕云),WU De-ling(吴德玲),JIN Chuan-shan(金传山),et al.Preliminary analysis on chemical components and content determination of total flavones in the Hull of Carya cathayensis Sarg.(山核桃外蒲壳化学成分初步分析及总黄酮的含量测定).J Anhui Coll Tradit Chin Med(安徽中医学院学报),2010,29(1):64.
[2] SHEN Bing-xiang(沈炳香),WU De-ling(吴德玲),CHEN Shi-yun(陈仕云),et al.Determination of total saponins in the Hull of Carya cathayensis Sarg.(山核桃外蒲壳总皂苷的含量测定).J Anhui Med Pharm(安徽医药),2009,13(5):513
[3] ZHANG Guo-hui(张国辉),MA Chen(马辰).Advances in studies on pharmacokinetics of flavonoids(黄酮类化合物药动学研究进展).Chin Tradit Herb Drugs(中草药),2004,35(5):582
[4] LI Shu-juan(李淑娟),ZHANG Li(张力),ZHANG Dan-shen(张丹参),et al.Experimental study on antisenility effect of chrysophanol(大黄酚抗衰老作用的实验研究).Chin J Geron(中国老年学杂志),2005,25(11):1362
[5] HAN Yuan-yuan(韩媛媛),WANG Li-yong(王利勇),DU Guang-ling(杜光玲),et al.HPLC-fluorescence determinatoin of rhein,emodin,chrysophanol in Dongle plaster(HPLC-荧光检测法测定东乐膏中大黄素、大黄酸、大黄酚的含量).Chin J Pharm Anal(药物分析杂志),2010,30(6):1016
[6] XU Qian-li(许乾丽),MAO Xiang-jun(茅向军),SONG Xiao-ning(宋晓宁),et al.HPLC simultaneous determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Liuwei Anxiao capsules(HPLC法同时测定六味安消胶囊中芦荟大黄素、大黄酸、大黄酚和大黄素甲醚的含量).Chin J Pharm Anal(药物分析杂志),2010,30(10):1841
[7] MA Zhe-long(马哲龙),JIANG Fu-sheng(蒋福升),YU Ting-ting(俞婷婷),et al.Determination on the content of DHMC from the leaf of Carya cathayensis Sarg.(山核桃树叶中球松素查耳酮的含量测定).China Med Her(中国医药导报),2011,22(8):69
[8] WU De-ling(吴德玲),CHEN Shi-yun(陈仕云),LIU Jing-song(刘劲松),et al.Chemical constituents of Carya cathayensis and their antitumor bioactivity(山核桃外蒲壳化学成分及抗肿瘤活性研究).J Chin Med Mater(中药材),2011,34(7):1055
[9] HUA Xiao-dong(华晓东),WANG Zhi-hua(王智华),WANG Ju-mei(王菊美),et al.Determination of rhien,emodin,and chrysophanol in Huangdanyinchen granules by HPLC(HPLC同时测定黄疸茵陈颗粒中大黄素、大黄酸、大黄酚的含量).Chin Tradit Pat Med(中成药),2002,24(5):340

欢迎阅读《药物分析杂志》!您是该文第 1308位读者!

药物分析杂志 © 2009
地址:北京天坛西里2号 邮政编码:100050; 电子邮件:ywfx@nicpbp.org.cn

本系统由北京博思汇文数字科技有限公司设计开发 技术服务电话:010-60213898