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期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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玉屏风颗粒2个异黄酮类及4个色原酮类成分的含量测定

HPLC simultaneous determination of two isoflavonoids, four chromones in Yupingfeng granules

分类号:
出版年·卷·期(页码):2012,32 (2):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立同时测定玉屏风颗粒中2个异黄酮类成分(毛蕊异黄酮葡萄糖苷、毛蕊异黄酮)及4个色原酮类成分(升麻素苷、升麻素、5-O-甲基维斯阿米醇苷、亥茅酚苷)含量的高效液相色谱法。 方法: 采用INERSTIL ODS3(4.6 mm×250 mm,5 μm)色谱柱,以乙腈-0.2%磷酸溶液为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长为254 nm。 结果: 升麻素苷、毛蕊异黄酮葡萄糖苷、升麻素、5-O-甲基维斯阿米醇苷、亥茅酚苷、毛蕊异黄酮线性范围分别为5.3~263.6,2.1~103.6,7.5~376.2,7.4~396.8,2.0~98.8,4.4~220.6 μg·mL-1(r≥0.9999);平均回收率(n=6)分别为99.9%,95.1%,95.7%,96.8%,101.7%,99.0%。 结论: 该法准确、可靠,可用于玉屏风颗粒的质量控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To develop an HPLC method for simultaneous determination of two isoflavonoids,(calycosin-7-O-β-D-glucoside,calycosin) and four chromones(prim-O-glucosylcimifugin,cimifugin,4'-O-β-glucopyranosyl-5-O-methylvisamminol,sec-O-glucosylhamaudol) in Yupingfeng granules. Methods: The six components were separated by INERSTIL ODS3(4.6 mm×250 mm,5 μm) column with gradient elution of acetonitrile-0.2% phosphoric acid as the mobile phase,the flow rate was 1.0 mL·min-1,and the detection wavelength was 254 nm. Results: The calibration curves were linear in the ranges of 5.3-263.6 μg·mL-1 for prim-O-glucosylcimifugin,2.1-103.6 μg·mL-1 for calycosin-7-O-β-D-glucoside,7.5-376.2 μg·mL-1 for cimifugin,7.4-396.8 μg·mL-1 for 4'-O-β-glucopyranosyl-5-O-methylvisamminol,2.0-98.8 μg·mL-1 for sec-O-glucosylhamaudol,4.4-220.6 μg·mL-1 for calycosin(r≥0.9999).The average recoveries(n=6) were 99.9%,95.1%,95.7%,96.8%,101.7%,99.0%,respectively. Conclusion: This method is simple,rapid and accurate with good reproducibility,it is suitable for the quality control of Yupingfeng granules.

-----参考文献:---------------------------------------------------------------------------------------
[1] ChP(中国药典).2010.Vol Ⅰ(一部):616
[2] LIU Rui(刘蕊).Advancement in research on pharmacology of flavonoids(黄酮类化合物的药理研究进展).Heilongjiang Med J(黑龙江医药),2010,23(2):234
[3] XUE Bao-yun(薛宝云),LI Wen(李文),LI Li(李丽).A pharmacodynamic research on chromone glucosides of Fangfeng(防风色原酮甙类成分的药理活性研究).China J Chin Mater Med(中国中药杂志),2000,25(5):297
[4] WANG Xin-zhi(王欣之),WEN Hong-mei(文红梅),LI Wei(李伟),et al.Simultaneous determination of four chromones,three flavonoids and two atractylenolqides in yupingfeng powder by HPLC(HPLC法同时测定玉屏风散中色原酮、黄酮和内酯类9种成分的含量).Tradit Chin Drug Res Clin Pharmacol(中药新药与临床药理),2009,20(2):142
[5] LI Kun-ping(李坤平),GAO Chong-kai(高崇凯),LI Wei-min(李卫民).UPLC-FMS analysis of water decoction and ultrasonic extract of Yupingfeng recipe(超高效液相色谱串联飞行时间质谱分析玉屏风方水煎煮和超声提取物).Chin J Pharm Anal(药物分析杂志),2010,30(10):1836
[6] ZHANG Li(张俐),WANG Yu(王玉),LI Zheng-yan(李争艳).HPLC-ELSD determination of astragaloside in Yupingfeng capsule(高效液相色谱-蒸发光散射检测法测定玉屏风胶囊中黄芪甲苷的含量).Chem Anal Meter(化学分析计量),2009,18(1):22
[7] HUANG Yue-chun(黄月纯),YIN Xue(尹雪),WEI Gang(魏刚).The fingerprint analysis on Yupingfeng decoction by HPLC(玉屏风汤剂的HPLC指纹图谱研究).Chin Tradit Pat Med(中成药),2008,30(10):1405

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