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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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新型键合纤维素手性固定相拆分氯西加酮对映体

Separation of losigamone enantiomers on a new cellulose bonded chiral stationary phase

分类号:
出版年·卷·期(页码):2012,32 (1):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立一种用新型键合纤维素手性固定相拆分氯西加酮对映异构体的高效液相色谱方法。方法: 使用Chiralpak IB(250 mm×4.6 mm,5 μm)色谱柱,流动相为正己烷-无水乙醇(90︰10),流速0.8 mL·min-1,检测波长230 nm,柱温30 ℃;通过对比氯西加酮和添加了苏式氯西加酮的氯西加酮色谱图,判断先流出物的构型。结果: 氯西加酮对映体在新型键合直链纤维素衍生化合物手性固定相上能够完全分离,分离度为1.79;先流出物为苏式氯西加酮。结论: 本方法可方便地实现氯西加酮对映体的分离。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To develop a liquid chromatographic method for the chiral separating enantiomers of losigamone. Methods: Losigamone enantiomers were separated by a Chiralpak IB(250 mm×4.6 mm,5 μm)at 30 ℃ and detected at 230 nm,the mobile phase used was n-hexane-ethanol(90:10)at a flow rate of 0.8 mL·min-1.By comparing the chromatograph of enantiomers of losigamone and the chromatograph of losigamone spiked with threo-losigamone,determine the structure of the first effluent. Results: Losigamone enantiomers were separated on amylase derivative chiral stationary phase and the resolution was 1.79.The first effluent is threo-losigamone. Conclusion: The present method can be used for separating the enantiomers of losigamone simply.

-----参考文献:---------------------------------------------------------------------------------------
[1] Borowicz KK,Kimber-Trojnar Z,Patsalos PN,et al.Isobolographic analysis of the interactions of losigamone,remacemide,zonisamide with conventional antiepileptic drugs in the maximal electroshock test in mice-preliminary report.Neurol Neurochir Pol,2003,3:35
[2] Srinivasan J,Richens A,Davies JA.The effect of losigamone(AO-33)on electrical activity and excitatory amino acid release in mouse cortical slices.Br J Pharmacol,1997,122:1490
[3] Zhang CL,Chatterjee SS,Stein U,et al.Comparison of the effects of losigamone and its isomers on maximal electroshock induced convulsions in mice and on three different patterns of low magnesium induced epileptiforn activity in slices of the rat temporal cortex.Naunyn-Schmiedeberg’s Arch Pharmacol,1992,345:85
[4] Kohr G,Heinemann U.Effects of the tetronic acid derivatives AO33(losigamone)and AO78 on epileptiform activity and on stimulus-induced calcium concentration changes in rat hippocampal slices.Epilepsy Res,1990,7:49
[5] Schmitz D,Gloveli T,Heinemann U.Effects of losigamone on synaptic potentials and spike frequency habituation in rat entorhinal cortex and hippocarnpal CA1 neurones.Neurosci Lett,1995,200:141
[6] Bialer M,Johannessen SI,Kupferberg HJ,et al.Progress report on new antiepileptic drugs:a summary of the fourth EILAT conference(EILAT IV).Epilepsy Res,1999,34:1
[7] Draguhn A,Jungclaus M,Sokolowa S,et al.Losigamone decreases spontaneous synaptic activity in cultured hippocampal neurons.Eur J Pharmacol,1997,325:245
[8] Baulac M,Klement S.Efficacy and safety of Losigamone in partial seizures:a randomized double-blind study.Epilepsy Res,2003,55:177
[9] Gebhardt C,Breustedt JM,Noldner M,et al.The antiepileptic drug losigamone decreases the persistent Na+ current in rat hippocampal neurons.Brain Res,2001,920:27
[10] Torchin CD,Yonekawa WD,Kapetanovic IM,et al.Chiral high-performance liquid chromatographic analysis of enantiomers of losigamone,a new candidate antiepileptic drug.J Chromatogr B,1999,724:101
[11] Peeters PA,Van Lier JJ,Van De Merbel N,et al.Pharmacokinetics of-labelled losigamone and enantiomers after oral administration to healthy subjects.Eur J Drug Metab Pharmacokinet,1998,23:45

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