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期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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水解蛋白注射液中游离氨基酸含量的测定及分析

Determination and evaluation of amino acids in protein hydrolysate injection

作者: 连莹, 仲平 
作者(英文):LIAN Ying, ZHONG Ping
分类号:
出版年·卷·期(页码):2012,32 (1):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立二硝基氟苯(DNFB)柱前衍生化法测定水解蛋白注射液中游离氨基酸含量,并对水解蛋白注射液质量进行分析。 方法: 采用DNFB为衍生化试剂进行衍生,色谱柱为Diamonsil C18(250 mm×4.6 mm,5 μm),流动相为0.05 mol·L-1醋酸钠溶液(含1% N,N-二甲基甲酰胺,调pH为6.4)与50%乙腈水溶液,梯度洗脱,流速1.0 mL·min-1,检测波长360 nm。 结果: 各氨基酸的线性关系良好;平均回收率(n=6)为98.7%~100.8%,RSD为0.46%~3.3%。 结论: 本研究建立了水解蛋白注射液游离氨基酸测定方法,为质量标准中游离氨基酸含量测定限度的制定提供了依据。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish a pro-column derivatization procedure for determination of the content of amino acid in protein hydrolysate injection by RP-HPLC. Methods: DNFB was used as a derivatization reagent and separation of DNP-amino acid derivatives were performed on Diamonsil C18 column(250 mm×4.6 mm,5 μm). The mobile phase was 0.05 mol·L-1 sodium acetate solution(containing 1% DMF and adjusted pH 6.4) and 50% acetonitrile with gradient elution at a flow rate of 1.0 mL·min-1, and the signals were detected at 360 nm. Results: Under the above condition, all amino acid in the standard solution were separated to baseline; The calibration curves of amino acids were in good linearity; The average recovery of amino acid ranged from 98.7% to 100.8% with RSD of 0.46%-3.3%(n=6). Conclusion: The method is applied to analyzing amino acid composition of protein hydrolysate injection and good results are obtained.

-----参考文献:---------------------------------------------------------------------------------------
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