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期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC法同时测定双黄连冻干粉中11个成分的含量

HPLC determination of eleven components in Shuanghuanglian freeze-dried powder

分类号:
出版年·卷·期(页码):2012,32 (1):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立同时测定双黄连冻干粉中11个成分(绿原酸、咖啡酸、芦丁、金丝桃苷、连翘酯苷、野黄芩苷、黄芩苷、连翘苷、木犀草素、黄芩素和汉黄芩素)含量的高效液相色谱法。 方法: 采用Agilent Zorbax SB-C18(250 mm×4.6 mm,5 μm)色谱柱,流动相为乙腈-0.1%甲酸水溶液,线性梯度洗脱,流速为1.0 mL·min-1;金丝桃苷、黄芩苷、连翘苷、黄芩素及汉黄芩素的检测波长为278 nm,绿原酸、咖啡酸、芦丁、连翘酯苷、野黄芩苷及木犀草素的检测波长为320 nm。 结果: 11个成分在考察的线性范围内,进样量与峰面积之间线性关系良好(r>0.9995);回收率(n=9)均在96.3%~104.0%范围内,RSD小于2.3%。 结论: 方法操作简单、准确,专属性强,适用于双黄连冻干粉的质量控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC method for determination of eleven compounds(chlorogenic acid, caffeic acid, rutin, hyperoside, forsythiaside, scutellarin, baicalin, forsythin, luteoloside, baicalein and wogonin) in Shuanghuanglian freeze-dried powder. Methods: Agilent Zorbax SB-C18 column(250 mm×4.6 mm, 5 μm) was adopted. The mobile phase was composed of acetonitrile and 0.1% formic acid water-solution in gradient elution mode. The flow rate was 1.0 mL·min-1. The detection wavelength for hyperoside, baicalin, forsythin, baicalein and wogonin was set at 278 nm, while that for chlorogenic acid, caffeic acid, rutin, forsythiaside, scutellarin and luteoloside was 320 nm. Results: The calibration curves were linear in certain ranges for eleven compounds(r>0.9995), respectively. All recoveries(n=9) were within the range of 96.3%-104.0% with RSD values less than 2.3%. Conclusion: The method is simple and accurate with high specificity. It can be used for the quality control of Shuanghuanglian freeze-dried powder.

-----参考文献:---------------------------------------------------------------------------------------
[1] ChP(中国药典).2010.Vol Ⅰ(一部):846
[2] ZHANG Ya-ming(张雅铭),YAN Dan(鄢丹),ZHANG Ping(张萍),et al.Quality control of Shuanghuanglian freeze-dried powder for injection based on its HPLC-ELSD fingerprints and biological profiles(基于化学特征图谱-生物热活性图谱关联检测的注射用双黄连冻干粉针质量控制方法的初步研究).Acta Pharm Sin(药学学报),2010,45(1):93
[3] HE Xin(何心),SHI Chun-wei(石春伟),LU Song-ping(芦松萍),et al. HPLC determination of chlorogenic acid and caffeic acid in Shuanghuanglian freeze-dried powder for injection(高效液相色谱法测定双黄连粉针中绿原酸和咖啡酸的含量).Chin J Pharm Anal(药物分析杂志),1997,17(6):410
[4] HUANG Yi-kun(黄义昆),LIANG Jian-cheng(梁健成),CAI Zhen(蔡震).Determination of baicalin and phillyrin in Shuanghuanglian powder by HPLC(高效液相色谱法同时测定双黄连粉针中黄芩苷和连翘苷含量).China Pharm(中国药业),2008,17(1):8

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