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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC法测定复方制剂布洛芬盐酸曲马多片中2个有效成分含量

Determination the content of two effective components in compound preparation of ibuprofen and tramadol hydrochloride tablets by HPLC

作者: 何连军, 张莉华 
作者(英文):HE Lian-jun, ZHANG Li-hua
分类号:R917
出版年·卷·期(页码):2020,40 (6):1113-1120
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立HPLC法测定复方制剂布洛芬盐酸曲马多片中2个有效成分布洛芬和盐酸曲马多的含量。方法: 采用Agilent ZORBAX Extend-C18色谱柱(150 mm×4.6 mm,5 μm),以乙腈-磷酸盐缓冲溶液(40:60)为流动相,流速1.0 mL·min-1,检测波长264 nm,柱温25℃,进样量20 μL,外标法定量。结果: 布洛芬和盐酸曲马多质量浓度分别在39.60~396.0 μg·mL-1r=1.000)和8.104~81.04 μg·mL-1r=1.000)范围内呈良好线性关系。布洛芬和盐酸曲马多的平均回收率(n=9)分别为99.8%、99.9%,RSD分别为1.1%、1.2%。结论: 所建立的方法简便、快速、准确,重复性好,可用于复方布洛芬盐酸曲马多片有效成分的测定。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To established an HPLC method for determining the content of ibuprofen and tramadol hydrochloride in compound preparation of ibuprofen and tramadol hydrochloride tablets. Methods: Agilent ZORBAX Extend-C18 column(150 mm×4.6 mm,5 μm)was adopted with the mobile phase of acetonitrile-phosphate buffer solution(40:60)at the flow rate of 1.0 mL·min-1;detection wavelength was 264 nm;column temperature was 25℃;injection volume was 20 μL.External standard method for quantitative analysis was used.Results: The calibration curves of ibuprofen and tramadol hydrochloride were in good linear relation in the ranges of 39.60-396.0 μg·mL-1(r=1.000),and 8.104-81.04 μg·mL-1(r=1.000).The average recoveries of ibuprofen and tramadol hydrochloride(n=9)were 99.8% and 99.9%,RSDs were 1.1% and 1.2% respectively.Conclusion: The method is simple,rapid,accurate and reproducible.It can be used to determine the effective components of compound ibuprofen and tramadol hydrochloride tablets.

-----参考文献:---------------------------------------------------------------------------------------
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