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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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应用分子印迹荧光传感器检测食品中的痕量腈菌唑

Determination of trace myclobutanil in foods by molecularly imprinted fluorescence sensor

分类号:R917
出版年·卷·期(页码):2020,40 (5):830-836
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:制备分子印迹荧光传感器(CDs@MIP),用于食品中痕量腈菌唑的分析。方法:以腈菌唑为模板分子,苯基三乙氧基硅烷(PTEOS)为功能单体,正硅酸乙酯(TEOS)为交联剂,采用溶胶-凝胶法在荧光碳点(CDs)表面包覆分子印迹层,制备分子印迹荧光传感器。腈菌唑可猝灭CDs@MIP的荧光,利用荧光强度与腈菌唑浓度相关的特性,测定腈菌唑的含量。结果:腈菌唑浓度在0.05~0.5 mg·kg-1范围内与CDs@MIP荧光猝灭率呈良好线性关系,检测下限为1.8 μg·kg-1,对3种样品中腈菌唑进行检测及加标回收实验。在大米中未检出腈菌唑,黄瓜与香蕉中腈菌唑残留量分别为0.82和0.074 mg·kg-1。样品回收率及RSD分别在95.3%~101.7%和2.9%~5.1%之间。结论:该传感器对腈菌唑具有较高灵敏度,以及良好的选择性,为实际样品中腈菌唑的简单﹑快速检测提供了新思路。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: The molecularly imprinted fluorescence sensor(CDs@MIP)was prepared and used for the analysis of trace amounts of myclobutanil in foods. Methods: A molecularly imprinted fluorescence sensor was prepared using myclobutanil,phenyltriethoxysilane(PTEOS)and tetraethyl orthosilicate(TEOS)as template molecule,functional monomer and cross-linking agent respectively,which polymerized and coated on the surface of fluorescent carbon dots(CDs)by sol-gel method. The content of myclobutanil was determined by the correlation between the fluorescence intensity and the concentration of myclobutanil because of the fluorescence quenching of CDs@MIP induced by myclobutanil. Results: The concentration of myclobutanil was in a linear relationship with the fluorescence quenching rate of CDs@MIP in the range of 0.05-0.5 mg·kg-1. The detection limit was 1.8 μg·kg-1. Meanwhile the content and recovery rate of myclobutanil in the three samples were tested. And myclobutanil in cucumber and banana was 0.82 and 0.074 mg·kg-1 and not detected in rice respectively. The recovery and the relative standard deviation(RSD)were 95.3%-101.7% and 2.9%~5.1% respectively. Conclusion: The prepared sensor had high sensitivity and good selectivity for myclobutanil,which provided a new idea for the simple and rapid detection of myclobutanil in practical samples.

-----参考文献:---------------------------------------------------------------------------------------
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