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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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在线固相萃取液相色谱-三重四极杆/线性离子阱质谱法同时测定人血浆中4种抗抑郁药的含量

Simultaneous determination of four antidepressants in human plasma by online solid-phase extraction LC-QTRAP-MS/MS

分类号:R917
出版年·卷·期(页码):2020,40 (5):821-829
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立在线固相萃取液相色谱-串联质谱同时测定人血浆中奥氮平、舍曲林、利培酮以及阿立哌唑4种抗抑郁药含量的检测方法。方法:用乙腈沉淀蛋白提取目标化合物,HySphere C18在线固相净化柱净化,CAPCELL PAK C18色谱柱分离,串联质谱采用电喷雾多反应监测正离子扫描模式进行检测,同位素内标法定量。结果:奥氮平、舍曲林、利培酮以及阿立哌唑4种抗抑郁药质量浓度在0.5~500 ng·mL-1范围内线性关系良好,相关系数(r)均大于0.998,3个加样水平下的平均回收率为90.0%~97.3%,RSD(n=6)为2.1%~9.9%,准确度为92.0%~102.8%,4种药物在患者样品中的检测下限为2 ng·mL-1结论:该方法简单,准确度高,灵敏度好,可用于奥氮平、舍曲林、利培酮和阿立哌唑血药浓度的快速同时测定。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To develop a new method using online solid-phase extraction (SPE) liquid chromatography combined with tandem mass spectrometry (MS/MS) for the determination of olanzapine,sertraline,risperidone and aripiprazole in human plasma. Methods: The plasma sample was precipitated with acetonitrile. The analytes in extract were on-line purified directly on HySphere C18 purification column where the sample matrices were washed away. Subsequently,the analytes which were eluted from the extraction column onto CAPCELL PAK C18 column were separated with a gradient elution,and detected with electrospray ionization mass spectrometry in the positive scan mode using multiple reaction monitoring. The isotope internal standards were employed for quantification. Results: The concentrations of olanzapine,sertraline,risperidone and aripiprazole were in the range of 0.5-500 ng·mL-1,respectively. The linear correlation coefficient(r)was greater than 0.998.Average recoveries of the analytes at three spiked concentration levels ranged from 90.0% to 97.3%,with relative standard deviations of 2.1% to 9.9%. The accuracy of four antidepressants was within the range of 92.0%-102.8%. The determination limit of four drugs in patient sample is 2 ng·mL-1. Conclusion: The method is simple,accurate and specific,and can be used for rapid simultaneous determination of plasma concentration of olanzapine,sertraline,risperidone and aripiprazole.

-----参考文献:---------------------------------------------------------------------------------------
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