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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC-DAD法同时测定九味痔疮胶囊中6个成分的含量

Simultaneous determination of 6 components in Jiuwei Zhichuang capsules by HPLC-DAD

分类号:R917
出版年·卷·期(页码):2020,40 (5):802-808
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立同时测定九味痔疮胶囊中鞣花酸、虎杖苷、表小檗碱、盐酸黄连碱、盐酸巴马汀、盐酸小檗碱6个成分含量的HPLC法。方法:通过盐酸-甲醇溶液(1∶99)水浴加热结合超声处理的方式对样品进行提取,采用XBridge C18(4.6 mm×250 mm,5 μm)色谱柱,以乙腈(A)-0.025 mol·L-1磷酸二氢钾溶液(B)为流动相进行梯度洗脱(0~30 min,15%A→50%A),流速为1.0 mL·min-1,柱温为25 ℃,进样量为10 μL,检测波长为345 nm。结果:测定的6个成分在所取质量浓度范围内均具有良好的线性关系(r≥0.999 8),精密度、稳定性和重复性良好,平均加样回收率为98.1%~98.8%,RSD为1.2%~2.0%。5批九味痔疮胶囊样品中测得的鞣花酸、虎杖苷、表小檗碱、盐酸黄连碱、盐酸巴马汀、盐酸小檗碱平均含量分别为1.10%、0.28%、0.25%、0.42%、0.33%、1.32%。结论:该方法操作简单,准确可靠,实用性强,可为完善九味痔疮胶囊的质量控制提供参考依据。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC method for simultaneous determination of 6 components including ellagic acid,polydatin,epiberberine,coptisine hydrochloride,palmatine hydrochloride,and berberine hydrochloride in Jiuwei Zhichuang capsules. Methods: The sample was extracted with ultrasonic treatment by hydrochloric acid-methanol solution (1:99) in heating water bath. XBridgeTM C18(4.6 mm×250 mm,5 μm)column was adopted. The gradient elution (0-30 min,15%A→50%A) was carried out with acetonitrile (A)-0.025 mol·L-1 potassium dihydrogen phosphate solution (B) as mobile phase. The flow rate was 1.0 mL·min-1, the column temperature was 25℃,the injection volume was 10 μL,and the detection wavelength was 345 nm. Results: The linear relationship of the 6 components was all excellent (r ≥ 0.999 8),and the results of precision,stability tests and repeatability were all good,the average recovery rates were 98.1%-98.8% with RSDs of 1.2%-2.0%. The average contents of ellagic acid,polydatin,epiberberine,coptisine hydrochloride,palmatine hydrochloride and berberine hydrochloride in 5 samples were 1.10%,0.28%,0.25%,0.42%,0.33% and 1.32%,respectively. Conclusion: This method is simple,accurate,reliable and practical. It can provide reference for improving the quality of Jiuwei Zhichuang capsules.

-----参考文献:---------------------------------------------------------------------------------------
[1] WS-10189(ZD-0189)-2002-2011Z国家中成药标准汇编外科妇科分册[S].2002 WS-10189(ZD-0189)-2002-2011Z Compilation of National Standard in Chinese Patent Medicine of Surgical and Gynecology[S].2002
[2] 孟凡水,刘睿,项维,等.肛泰软膏联合九味痔疮胶囊治疗痔疮疗效观察研究[J].海峡药学,2016,28(4):212 MENG FS,LIU R,XIANG W,et al.Observation on the therapeutic effect of Gangtai ointment combined with Jiuwei Zhichuang capsule in treating hemorrhoids[J].Strait Pharm J,2016,28(4):212
[3] 韩奇亨,张春红, 龚雪,等.鞣花酸类化合物在野牡丹科植物中的分布与药理活性研究[J].中药材,2018,41(12):2962 HAN QH,ZHANG CH,GONG X,et al.Distribution and pharmacological activity of ellagic acid compounds in wild peony plants[J].J Chin Med Mater,2018,41(12):2962
[4] 王亚运,张琪.虎杖苷的药理作用研究进展[J].医学综述,2017,23(5):989 WANG YY,ZHANG Q.Research progress of polydatin pharmacologic actions[J].Med Recapit,2017,23(5):989
[5] TAN LH,LI CL,SU ZR,et al.Epiberberine,a natural protoberberine alkaloid,inhibits urease of Helicobacter pylori and jack bean:susceptibility and mechanism[J].Eur J Pharm Sci,2017,110(15):77
[6] 韦晟,刘金春,葛卫红.黄连碱对过氧化氢损伤的血管内皮细胞保护作用[J].中国医药导报,2019,16(3):4 WEI S,LIU JC,GE WH.Protective effect of coptisine on vascular endothelial cells injured by hydrogen peroxide[J].China Med Her,2019,16(3):4
[7] 宁娜.巴马汀降脂活性及作用机理研究[D].重庆:西南大学,2015 NING N.Study on Hypolipidemic Effect of Palmatine from Coptis chinensis and Its Possible Mechanism[D].Chongqing:Southwest University,2015
[8] 李东伟,邵梦寒,葛传慧,等.小檗碱调节糖脂代谢及其抗糖尿病活性的研究[J].生命科学,2019,31(2):201 LI DW,SHAO MH,GE CH,et al.Study of berberine regulating gene expression of glucolipid metabolism and its anti-diabetic activities[J].Chin Bull Life Sci,2019,31(2):201
[9] 魏小聪,赵艳敏,崔雨,等.HPLC法测定树莓叶和果实中鞣花酸的含量[J].武警后勤学院学报(医学版),2016,25(1):29 WEI XC,ZHAO YM,CUI Y,et al.Determination of ellagic acid content in raspberry leaves and fruits by HPLC[J].J Log Univ PAP(Med Sci),2016,25(1):29
[10] 王玮,陈扣宝,胡云飞,等.蛇莓饮片质量标准研究[J].中南药学,2019,17(5):730 WANG W,CHEN KB,HU YF,et al.Quality standard for Duchesneae Indicae Herba[J].Cent South Pharm,2019,17(5):730
[11] 翟迎春,金欣,秦亚东,等.HPCE法同时测定黄连中四种生物碱含量[J].现代中药研究与实践,2015,29(5):24 ZHAI YC,JIN X,QIN YD,et al.simultaneous determination of four alkaloids components in Coptidis Rhizoma by HPCE[J].Chin Med J Res Pract,2015,29(5):24
[12] 蔺红伟,江春霞,朴淑娟,等.用HPLC法测定不同地区虎杖饮片中的4种有效成分[J].药学服务与研究,2017,17(1):27 LIN HW,JIANG CX,PIAO SJ,et al.Determination of four active ingredients in Polygonum cuspidatum from different regions by HPLC method[J].Pharm Care Res,2017,17(1):27

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