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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC-DAD法同时测定栀子金花丸中10个成分的含量

Simultaneous determination of ten components in Zhizi Jinhua pills by HPLC-DAD

分类号:R917
出版年·卷·期(页码):2020,40 (1):145-154
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立HPLC-DAD法同时测定栀子金花丸中栀子苷、绿原酸、黄芩苷、芒果苷、小檗碱、黄柏碱、大黄酸、大黄素、芦荟大黄素和大黄酚10个成分的含量。方法: 采用Luna C18色谱柱(4.6 mm×250 mm,5 μm),以乙腈-0.2%醋酸水溶液为流动相,梯度洗脱(0~15 min,8% A→40% A;15~20 min,40% A→60% A;20~30 min,60% A→90% A;30~40 min,90% A→8% A),流速1.0 mL·min-1,柱温35℃,检测波长258 nm(检测栀子苷、芒果苷、大黄酸、大黄素、芦荟大黄素和大黄酚),280nm(检测黄芩苷和黄柏碱),345 nm(检测绿原酸和小檗碱)。结果: 栀子苷、绿原酸、黄芩苷、芒果苷、小檗碱、黄柏碱、大黄酸、大黄素、芦荟大黄素和大黄酚10个待测成分实现完全分离,并与其他成分达到良好的分离;上述成分线性范围分别为0.66~16.43 μg·mL-1r=0.999 7)、0.42~10.45 μg·mL-1r=0.999 2)、0.30~7.50 μg·mL-1r=0.995 7)、0.64~15.95 μg·mL-1r=0.996 5)、0.59~15.06 μg·mL-1r=0.999 6)、0.06~1.59 μg·mL-1r=0.998 3)、0.27~6.78 μg·mL-1r=0.993 8)、0.07~1.82 μg·mL-1r=0.989 0)、0.04~1.03 μg·mL-1r=0.995 2)和0.10~2.53 μg·mL-1r=0.999 8);平均加样回收率(n=9)分别为100.4%、99.7%、99.0%、99.5%、99.2%、97.8%、97.9%、97.8%、97.8%和99.1%。5批样品中上述10个成分的含量范围分别为3.45~3.72、2.30~2.36、1.68~1.75、3.46~3.58、3.48~3.57、0.34~0.41、1.46~1.55、0.42~0.47、0.22~0.26、0.50~0.56 mg·g-1结论: 本法可为栀子金花丸的质量控制和考察提供参考。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC method for simultaneous determination of geniposide,chlorogenic acid,baicalin,mangiferin,berberine,phellodendrine,rhein,emodin,aloe emodin and chrysophanol in Zhizi Jinhua pills. Methods: HPLC separation was performed on a Luna C18 column(4.6 mm×250 mm,5 μm),with the mobile phase consisting of acetonitrile-0.2% acetic acid aqueous solution at a flow rate of 1.0 mL·min-1 in a gradient elution mode(0-15 min,8%A→40%A;15-20 min,40%A→60%A;20-30 min,60%A→90%A;30-40 min,90%A→8%A). The column temperature was at 35℃ and the detection wavelengths were set at 258 nm for geniposide,mangiferin,rhein,emodin,aloe emodin,and chrysophanol,280 nm for baicalin and phellodendrine,and 345 nm for chlorogenic acid and berberine. Results: Geniposide,chlorogenic acid,baicalin,mangiferin,berberine,phellodendrine,rhein,emodin,aloe emodin and chrysophanol were completely separated,and well separated from other constituents. The linear ranges of above-mentioned components were 0.66-16.43 μg·mL-1(r=0.999 7),0.42-10.45 μg·mL-1(r=0.999 2), 0.30-7.50 μg·mL-1(r=0.995 7),0.64-15.95 μg·mL-1(r=0.996 5),0.59-15.06 μg·mL-1(r=0.999 6),0.06-1.59 μg·mL-1(r=0.998 3),0.27-6.78 μg·mL-1(r=0.993 8),0.07-1.82 μg·mL-1(r=0.989 0),0.04-1.03 μg·mL-1(r=0.995 2)and 0.10-2.53 μg·mL-1(r=0.999 8),respectively. The average recoveries were 100.4%,99.7%,99.0%,99.5%,99.2%,97.8%,97.9%,97.8%,97.8% and 99.1%,respectively. The content ranges of ten ingredients were 3.45-3.72 mg·g-1,2.30-2.36 mg·g-1,1.68-1.75 mg·g-1,3.46-3.58 mg·g-1,3.48-3.57 mg·g-1,0.34-0.41 mg·g-1, 1.46-1.55 mg·g-1,0.42-0.47 mg·g-1,0.22-0.26 mg·g-1 and 0.50-0.56 mg·g-1,respectively. Conclusion: The established method provides references for the quality control and evaluate of Zhizi Jinhua pills.

-----参考文献:---------------------------------------------------------------------------------------

[1] 中华人民共和国药典2015年版.一部[S].2015:1538 ChP 2015.Vol Ⅰ[S].2015:1538
[2] 肖培根,李大鹏,杨世林.新编中药志.第三卷[M].北京:化学工业出版社,2002 XIAO PG,LI DP,YANG SL.Modern Chinese Materia Medica.Vol Ⅲ[M].Beijng:Chemical Industry Press,2002
[3] 曲蕃升,邢丽媛,许丽娜.栀子金花丸多指标成分HPLC含量测定方法研究[J].现代中药研究与实践,2017,31(1):56 QU FS,XING LY,XU LN.Multiple compounds determination of Zhizi-Jinhua pills by high-performance liquid chromatography[J].Res Pract Chin Med,2017,31(1):56
[4] 赵倩,冯伟红,张启伟,等."一测多评"法用于栀子金花丸多成分含量测定的可行性研究[J].中国中药杂志,2014,39(10):1826 ZHAO Q,FENG WH,ZHANG QW,et al.Feasibility study of QAMS for quantitative analysis of multiple structural types of ingredients in Zhizi Jinhua pill[J].China J Chin Mater Med,2014,39(10):1826
[5] 陈帅,王慧竹,薛健飞,等.栀子金花丸HPLC指纹图谱及其与体外抗氧化活性的相关性分析[J].中国药房,2016,27(22):3077 CHEN S,WANG HZ,XUE JF,et al.Analysis of the relationship of HPLC fingerprint of Zhizi Jinhua pills with its in vitro antioxidant activity[J].China Pharm,2016,27(22):3077
[6] 黎晓玲,彭瑞光.HPLC同时测定栀子金花丸中4种成分的含量[J].安徽农业科学,2014,42(6):1665 LI XL,PENG RG.Simultaneous determination of 4 components in Zhizijinhua pill by HPLC[J].J Anhui Agric Sci,2014,42(6):1665
[7] 蒋范任,蔡洪鲲,周翀,等.反相高效液相色谱法同时测定栀子金花丸中栀子苷、小檗碱、黄芩苷、大黄酸、大黄素、大黄酚的含量[J].中南药学,2015,13(2):172 JIANG FR,CAI HK,ZHOU C,et al.Determination of geniposide,berberine,baicalin,rhein,emodin and chrysophanol in Zhizi Jinhua pill by RP-HPLC[J].Cent South Pharm,2015,13(2):172
[8] 高晓燕,卢建秋.栀子金花丸中栀子苷、黄芩苷、番泻苷A和番泻苷B的含量测定方法研究[J].中国中药杂志,2009,34(20):2649 GAO XY,LU JQ.Study on determination of geniposide,baicalin,sennoside A and sennoside B in Zhizijinhua Wan[J].China J Chin Mater Med,2009,34(20):2649
[9] 温金莲,周清,宋粉云.RP-HPLC测定栀子金花丸中芒果苷的含量[J].中国实验方剂学杂志,2011,17(17):65 WEN JL,ZHOU Q,SONG FY.Determination of mangiferin in Zhizi Jinhua Wan by RP-HPLC[J].Chin J Exp Tradit Med Form,2011,17(17):65
[10] 郑峰,徐成志.HPLC法测定栀子金花丸中大黄素和大黄酚的含量[J].安徽医药,2007,1(11):1006 ZHENG F,XU CZ.Determination of emodin and chrysophanol in Zhizijinhua Wan by HPLC[J].Anhui Med Pharm J,2007,1(11):1006
[11] 何昱,周惠芬,黄丽娜,等.HPLC-DAD法同时测定谷红注射液中7个组分的含量[J].药物分析杂志,2015,35(6):954 HE Y,ZHOU HF,HUANG LN,et al.Simultaneous quantification of seven components in Guhong injection by HPLC-DAD[J].Chin J Pharm Anal,2015,35(6):954
[12] 郭小龙,蔡敏,陈勇,等.液相色谱法同时测定栀子金花丸中栀子苷和盐酸小檗碱的含量[J].湖北大学学报(自然科学版),2007,29(3):283 GUO XL,CAI M,CHEN Y,et al.Simultaneous determination of geniposide and berberine hydrochloride in Zhizijinhua Wan by high performance liquid chromatography[J].J Hubei Univ(Nat Sci),2007,29(3):283
[13] 王常顺,刘永利,段吉平,等.双波长-UPLC法同时测定知柏地黄丸中6个成分的含量[J].药物分析杂志,2018,38(2):256 WANG CS,LIU YL,DUAN JP,et al.Simultaneous quantification of six components in Zhibai Dihuang pills by dual-wavelength UPLC[J].Chin J Pharm Anal,2018,38(2):256
[14] 郝乘仪,于蕾,昌盛,等.HPLC-DAD法同时测定栀芩清热合剂中多指标成分含量[J].药物分析杂志,2018,38(9):1601 HAO CY,YU L,CHANG S,et al.Simultaneous determination of index compounds in Zhiqin Qingre mixture by HPLC-DAD[J].Chin J Pharm Anal,2018,38(9):1601
[15] 陈晓虎,苏晶,王慧,等.UPLC法同时测定栀子金花丸中11种成分[J].中草药,2014,45(6):955 CHEN XH,SU J,WANG H,et al.Simultaneous determination of 11 components in Zhizijinhua pill by UPLC[J].China Tradit Herb Drugs,2014,45(6):955

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