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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC法同时测定真菌竹黄胶囊中3个醌类成分的含量

Simultaneous determination of three perylenequinones in Zhenjun Zhuhuang capsules by HPLC

分类号:R917
出版年·卷·期(页码):2020,40 (1):139-144
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立同时测定真菌竹黄胶囊中竹红菌丙素、竹红菌甲素、竹红菌乙素3个苝醌类成分含量的高效液相色谱法。方法: 采用CAPCELL PAK C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈-0.1%磷酸溶液(62:38)为流动相,流速1.0 mL·min-1,检测波长460 nm,柱温30℃。结果: 竹红菌丙素、竹红菌甲素、竹红菌乙素进样量分别在0.016~0.816 μg(r=0.999 9)、0.020~0.982 μg(r=0.999 9)、0.040~2.016 μg(r=0.999 9)范围内与峰面积呈现良好的线性关系;平均回收率(n=3)分别为98.4%~98.6%(RSD<1.2%)、100.4%~100.8%(RSD<0.6%)、97.9%~100.2%(RSD<1.0%);3批样品中竹红菌丙素、竹红菌甲素、竹红菌乙素含量范围分别为0.434~0.441、0.661~0.674、1.308~1.323 mg·粒-1结论: 该方法可用于真菌竹黄胶囊中竹红菌丙素、竹红菌甲素、竹红菌乙素的含量测定。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC method for the simultaneous determination of three perylenequinones(hypocrellin C,hypocrellin A and hypocrellin B)in Zhenjun Zhuhuang capsules. Methods: The CAPCELL PAK C18(250 mm×4.6 mm,5 μm)was used with the mobile phase of acetonitrile-0.1% phosphoric acid solution(62:38)at a flow rate of 1.0 mL·min-1. The detection wavelength was 460 nm and the column temperature was 30℃. Results: The linear ranges of hypocrellin C,hypocrellin A and hypocrellin B were 0.016-0.816 μg(r=0.999 9), 0.020-0.982 μg(r=0.999 9)and 0.040-2.016 μg(r=0.999 9),respectively. The average recoveries(n=3)were 98.4%-98.6%(RSD<1.2%),100.4%-100.8%(RSD<0.6%)and 97.9%-100.2%(RSD<1.0%),respectively. The contents of hypocrellin C,hypocrellin A and hypocrellin B in three batches of samples were 0.434-0.441,0.661-0.674,1.308-1.323 mg per capsule,respectively. Conclusion: The method can be used to determine contents of hypocrellin C,hypocrellin A and hypocrellin B in Zhenjun Zhuhuang capsules.

-----参考文献:---------------------------------------------------------------------------------------

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