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期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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miRNA检测方法的研究进展

Research progress of microRNAs detection methods

分类号:R917
出版年·卷·期(页码):2020,40 (1):70-82
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

miRNA在基因调控方面起着重要作用,而其异常表达与多种疾病相关,其异常和特异性的表达提示其可成为诊断或预后的生物标志物。因此准确测定其表达水平对于其应用十分关键。但是由于miRNA序列短,同源序列相似度高,含量低,缺乏能够使其选择性扩增的共同特征,使得检测miRNA十分具有挑战性。本文对近年来miRNA的检测方法进行了概述,包括传统方法及其改进进展,以及一些新的检测方法,并对这些方法的优缺点进行了总结。

-----英文摘要:---------------------------------------------------------------------------------------

MicroRNAs(miRNAs) play a vital role in regulating gene expression and their distorted expression is associated with a variety of diseases. Their distorted and unique expression suggested that they could be potential markers in clinical diagnoses and prognoses. Thus,accurate determination of miRNA expression level is a prerequisite for their applications. The detection of miRNA is very challenging because of its small molecular size,low abundance and similar nucleotide sequences among its family members. In this review,we present an overview of miRNA detection methods in recent years. Methods that can be employed in routine clinic diagnostics was focused,including their advantages as well as their shortcomings,with special focus on the most innovative ones.

-----参考文献:---------------------------------------------------------------------------------------

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