关闭
 
读者在线:用户名 密码
首页 期刊简介 投稿须知 期刊目录 专家风采 编委会 特邀顾问 联系我们 移动出版
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5



刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

访问统计
您是第  4 8 5 1 4 1 4 位浏览者
您当前的位置:首页 >> 正文

重组人1型单纯疱疹病毒样品的活性检测研究

Biological activity determination of recombinant human herpes simplex virus type 1

分类号:R917
出版年·卷·期(页码):2020,40 (1):20-30
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立分析重组人1型单纯疱疹病毒(rHSV-1)样品感染滴度和生物学活性的方法。方法: 采用噬斑法检测重组人1型单纯疱疹病毒的感染滴度,采用选择性增殖活性检测法和肿瘤细胞杀伤活性检测法检测样品的生物学活性,分别对上述方法进行重复性和精密度性能验证,并用上述方法对6批样品中的感染滴度和生物学活性进行检测。结果: 经验证,上述3种方法均有较好的重复性和精密度,精密度RSD分别为5.3%、3.9%和19.4%;6批测试样品中,感染滴度为(3.06±0.37)×107 PFU·mL-1,选择性增殖活性为(1.58±0.63)×105,肿瘤细胞杀伤活性为0.027 8±0.015 9。结论: 经方法学验证,噬斑法、选择性增殖活性检测法和肿瘤细胞杀伤活性检测法可分别用于重组人1型单纯疱疹病毒的感染滴度和生物学活性的检测。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To develop methods to determine the biological activity and infectious titer of recombinant human herpes simplex virus type 1(rHSV-1) samples. Methods: The titer of rHSV-1 was determined by plaque assay, and the biological activity of rHSV-1 was determined by selective proliferative assay and tumoricidal assay. All the methods were validated in repeatability and precision, and used to detect the titers and biological activities of 6 batches of rHSV-1 samples. Results: The developed methods showed good repeatability and precision, with inter-assay precision being 5.3%, 3.9% and 19.4%, separately. Six batches of rHSV-1 were determined, with the average titer being (3.06±0.37)×107 PFU·mL-1, the average selective proliferative activity being (1.58±0.63)×105, and the average tumoricidal activity being 0.027 8±0.015 9. Conclusion: The developed methods are validated to be viable for the determination of titer and biological activity of rHSV-1.

-----参考文献:---------------------------------------------------------------------------------------

[1] LEDFORD H.Cancer-fighting viruses win approval[J].Nature, 2015, 526(7575):622
[2] KAUFMAN HL, KOHLHAPP FJ, ZLOZA A.Oncolytic viruses:a new class of immunotherapy drugs[J].Nat Rev Drug Discov, 2015, 14(9):642
[3] 喻启桂.肿瘤治疗的新突破:溶瘤病毒治疗[J].安徽医药, 2018, 22(1):1 YU QG.New breakthrough in cancer treatment:oncolytic virus therapy[J].Anhui Med Pharm J, 2018, 22(1):1
[4] SHERIDAN C.First oncolytic virus edges towards approval in surprise vote[J].Nat Biotechnol, 2015, 33(6):569
[5] COFFIN R.Interview with Robert Coffin, inventor of T-VEC:the first oncolytic immunotherapy approved for the treatment of cancer[J].Immunotherapy, 2016, 8(2):103
[6] MATTILA RK, HARILA K, KANGAS SM, et al.An investigation of herpes simplex virus type 1 latency in a novel mouse dorsal root ganglion model suggests a role for ICP34.5 in reactivation[J].J Gen Virol, 2015, 96(8):2304
[7] LIPSON EJ, SHARFMAN WH, CHEN S, et al.Safety and immunologic correlates of Melanoma GVAX, a GM-CSF secreting allogeneic melanoma cell vaccine administered in the adjuvant setting[J].J Transl Med, 2015, 13(1):214
[8] KOHLHAPP FJ, KAUFMAN HL.Molecular pathway:mechanism of action for talimogene laherparepvec, a new oncolytic virus immunotherapy[J].Clin Cancer Res, 2016, 22(5):1048
[9] Liu BL, Robinson M, Han ZQ, et al.ICP34.5 deleted herpes simplex virus with enhanced oncolytic, immune stimulating, and anti-tumour properties[J].Gene Ther, 2003, 10(4):292
[10] Coffey MC, Strong JE, Forsyth PA, et al.Reovirus therapy of tumors with activated Ras pathway[J].Science, 1998, 282(5392):1332
[11] 高凯, 付志浩, 李永红, 等.重组复制型溶瘤单纯疱疹病毒人细胞巨噬细胞集落刺激因子的质量研究[J].中国药学杂志, 2011, 46(19):1520 GAO K, FU ZH, LI YH, et al.Quality control research on recombinant oncolytic herpes simplex virus serotype 1 encoding human GM-CSF gene[J].Chin Pharm J, 2011, 46(19):1520
[12] YANG TT, SINAI P, KAIN SR.An acid phosphatase assay for quantifying the growth of adherent and nonadherent cells[J].Anal Biochem, 1996, 241(1):103
[13] 杨海智, 马海霞, 杨信东.国内关于半数致死量及类似生物效应指标测算方法研究进展[J].国外医药抗生素分册, 2012, 33(2):62 YANG HZ, MA HX, YANG XD.Current research progress on the calculation methods of LD50 and similar biological effect indicators[J].World Notes Antibiot, 2012, 33(2):62
[14] LI S, WANG F, ZHAI Z, et al.Synergistic effect of bladder cancer-specific oncolytic adenovirus in combination with chemotherapy[J].Oncol Lett, 2017, 14(2):2081
[15] KURYK L, MOLLER AW, JADERBERG M.Combination of immunogenic oncolytic adenovirus ONCOS-102 with anti-PD-1 pembrolizumab exhibits synergistic antitumor effect in humanized A2058 melanoma huNOG mouse model[J].Oncoimmunology, 2018, 8(2):e1532763
[16] JOO KJ, LI H, ZHANG X, et al.Therapeutic effect on bladder cancer with a conditionally replicating oncolytic virus derived from type Ⅱ herpes simplex virus[J].Bladder Cancer, 2015, 1(1):81
[17] FU X, TAO L, WANG PY, et al.Comparison of infectivity and spread between HSV-1 and HSV-2 based oncolytic viruses on tumor cells with different receptor expression profiles[J].Oncotarget, 2018, 9(30):21348
[18] YE T, JIANG K, WEI L, et al.Oncolytic Newcastle disease virus induces autophagy-dependent immunogenic cell death in lung cancer cells[J].Am J Cancer Res, 2018, 8(8):1514
[19] ABDULLAHI S, JAKEL M, BEHREND SJ, et al.A novel chimeric oncolytic virus vector for improved safety and efficacy as a platform for the treatment of hepatocellular carcinoma[J].J Virol, 2018, 92(23):doi:10.1128/JVI.01386-18
[20] LIU Z, RAVINDRANATHAN R, KALINSKI P, et al.Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy[J].Nat Commun, 2017, 8:14754
[21] CHON HJ, LEE WS, YANG H, et al.Tumor microenvironment remodeling by intratumoral oncolytic vaccinia virus enhances the efficacy of immune-checkpoint blockade[J].Clin Cancer Res, 2019, 25(5):1612
[22] HWANG CC, IGASE M, SAKURAI M, et al.Oncolytic reovirus therapy:pilot study in dogs with spontaneously occurring tumours[J].Vet Comp Oncol, 2018, 16(2):229
[23] IGASE M, SHOUSU K, FUJIKI N, et al.Anti-tumour activity of oncolytic reovirus against canine histiocytic sarcoma cells[J].Vet Comp Oncol, 2019, 17(2):184
[24] ZEYAULLAH M, PATRO M, AHMAD I, et al.Oncolytic viruses in the treatment of cancer:a review of current atrategies[J].Pathol Oncol Res, 2012, 18(4):771
[25] DAI MH, ZAMARIN D, GAO SP, et al.Synergistic action of oncolytic herpes simplex virus and radiotherapy in pancreatic cancer cell lines[J].Br J Surg, 2010, 97(9):1385
[26] WATANABE D.Medical application of herpes simplex virus[J].J Dermatol Sci, 2010, 57(2):75
[27] 张静, 李伟华, 陈顺英, 等.用蚀斑法进行麻疹疫苗的病毒滴定[J].微生物学免疫学进展, 1999, 27(1):35 ZHANG J, LI WH, CHEN SY, et al.Plaque assay for the titration of measles vaccine[J].Prog Microbiol Immunol, 1999, 27(1):35

欢迎阅读《药物分析杂志》!您是该文第 194位读者!

药物分析杂志 © 2009
地址:北京天坛西里2号 邮政编码:100050; 电子邮件:ywfx@nicpbp.org.cn

本系统由北京博思汇文数字科技有限公司设计开发 技术服务电话:400-921-9838