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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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中药材二氧化硫残留快速检测试剂盒的建立及应用

Establishment and application of a rapid test kit for determination of sulfur dioxide residues in Chinese herbal medicines

作者(英文):
分类号:R917
出版年·卷·期(页码):2019,39 (12):2191-2198
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立一种中药材二氧化硫残留的半定量快速检测试剂盒。方法:利用气体法对亚硫酸钠标准品进行检测,确定敏感度及检测线性范围,建立二氧化硫残留检测试剂盒;用试剂盒与2015年版《中华人民共和国药典》第三法(离子色谱法)同时对74种不同批次市售中药材的二氧化硫残留量进行检测比较,评价试剂盒的应用效果。结果:二氧化硫残留快速检测试剂盒敏感度为10 mg·kg-1,检测范围10~750 mg·kg-1,稳定性至少可达12个月;应用74种不同批次市售中药材验证,试剂盒检测结果与2015年版《中华人民共和国药典》第三法检测结果一致。结论:该试剂盒准确性好,稳定可靠,操作简便,可用于中药材二氧化硫残留的半定量快速检测。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish a semi-quantitative rapid test kit for determination of sulfur dioxide residues in Chinese herbal medicine. Methods: The sensitivity and linear range were determined through tested the standard product of sodium sulfite by gas method,and the test kit for sulfur dioxide residues was established. The sulfur dioxide residues of 74 different batches of commercially available Chinese herbal medicines were measured and compared by the kit and the third method (ion chromatography) of the 2015 edition of Chinese Pharmacopoeia,and the application effect of the kit was evaluated. Results: The sensitivity of sulfur dioxide residue rapid detection kit was 10 mg·kg-1,the detection range was 10-750 mg·kg-1,and the stability could reach at least 12 months. The accuracy of the kit was verified using 74 different batches of commercially available Chinese herbal medicines,the test results of the kit is consistent with those of the third method of the 2015 edition of the Chinese Pharmacopoeia. Conclusion: The kit is accurate,stable and reliable,and easy to operate. It can be used for semi-quantitative rapid detection of sulfur dioxide residues in Chinese herbal medicine.

-----参考文献:---------------------------------------------------------------------------------------

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