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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC法同时测定刘寄奴中5个成分的含量

Simultaneous determination of five components in Herba Artemisiae Anomalae by HPLC

分类号:R917
出版年·卷·期(页码):2019,39 (11):1998-2002
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立HPLC法同时测定刘寄奴中槲皮素、柚皮素、山柰酚、芒柄花素、异泽兰黄素5个成分的含量。方法:采用Syncronis C18色谱柱(250 mm×4.6 mm,5 μm),流动相为甲醇-0.2%磷酸水(40:60),检测波长260 nm,流速1.0 mL·min-1,柱温25℃,进样量10 μL。结果:将实验测得的图谱与标准物质进行对照,确定其5个成分分别为槲皮素、柚皮素、山柰酚、芒柄花素、异泽兰黄素;5个成分分别在测定范围内(0.062~0.992 μg,0.031~0.496 μg,0.034~0.544 μg,0.022~0.352 μg,0.027~0.432 μg)线性关系良好;平均加样回收率为97.8%~100.6%,RSD均小于3.0%;4批样品中5个成分的含量分别为0.022%~0.041%、0.009%~0.024%、0.011%~0.023%、0.006%~0.014%、0.017%~0.033%。结论:该方法简便准确,重复性好,可用于刘寄奴5个成分的含量测定。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC method for simultaneous determination of five components in Herba Artemisiae Anomalae.Methods: The HPLC assay was performed on a Syncronis C18 (250 mm×4.6 mm, 5 μm) column.The mobile phase was methanol-0.2% phosphoric acid (40:60).The flow rate was 1 mL·min-1, the detection wavelength was 260 nm, the column temperature was 25℃ and the injection volume was 10 μL.Results: Five constituents of quercetin, naringin, kaempferol, formononetin and eupatilin were identified by comparing with the spectra of standard substances.The linear relationship of the five components was good within the range (0.062-0.992 μg, 0.031-0.496 μg, 0.034-0.544 μg, 0.022-0.352 μg and 0.027-0.432 μg).The average recoveries of sample addition were in the range of 97.8%-100.6% (RSD<3.0%).The contents of five components in four batches of samples were 0.022%-0.041%, 0.009%-0.024%, 0.011%-0.023%, 0.006%-0.014% and 0.017%-0.033%. Conclusion: The method is simple, accurate and reproducible.It can be used for the determination of five components in Herba Artemisiae Anomalae.

-----参考文献:---------------------------------------------------------------------------------------

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