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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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药品质量控制中常见产毒真菌ITS和LSU rRNA基因的序列分析

Evaluation on ITS and LSU ribosomal RNA gene sequences as targets for phylogenetic analysis of mycotoxigenic fungi in pharmaceutical quality control

分类号:R917
出版年·卷·期(页码):2019,39 (11):1933-1939
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:评价rRNA基因的内转录间隔区(internal transcribed spacer,ITS)和大亚基单元(large subunit,LSU)序列对药品质量控制中常见产毒真菌的鉴定作用。方法:以形态分类方法对8个产毒真菌属的菌株进行初步鉴定;然后从试验菌株中提取基因组DNA,对ITS和LSU序列进行PCR扩增和序列测定,通过序列聚类关系分析,进一步评价不同序列对产毒真菌的分子鉴定能力。结果:分别采用ITS、LSU rRNA基因序列分析方法对产毒真菌的鉴定至少可达到"属"水平,采用ITS和LSU rRNA基因的串联序列可以将青霉属和曲霉属中的试验菌株鉴定至"种"水平。结论:ITS和LSU rRNA序列系统发育分析可作为产毒真菌的鉴定方法。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To identify mycotoxigenic fungi in pharmaceutical quality control based on sequence analysis of internal transcribed spacer (ITS) region and partial large subunit (LSU) of ribosomal RNA. Methods: Eight genus strains were firstly confirmed by morphological identification. The genomic DNAs were extracted, the ITS and LSU regions were amplified by polymerase chain reaction (PCR) and the PCR products were sequenced. Molecular identification capacity of different target sequences for mycotoxigenic fungi was evaluated by phylogenetic relationship analysis. Results: The ITS and LSU sequences could successfully identified mycotoxigenic fungi to genus-level at least. The compilation of ITS and LSU sequences could accurately identify Penicillium and Aspergillus. Conclusion: The phylogenetic analysis of the ITS and LSU sequence can provide the important reference method for the identification of mycotoxigenic fungi.

-----参考文献:---------------------------------------------------------------------------------------

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