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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nicpbp.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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HPLC-MS/MS法同时测定不同桂花品种中7个多酚类活性成分的含量

Simultaneous determination of seven active polyphenols in different Osmanthus fragrans cultivars by HPLC-MS/MS

分类号:R917
出版年·卷·期(页码):2019,39 (10):1811-1820
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立同时测定3个桂花品种丹桂、金桂、银桂中7个多酚类活性成分(绿原酸、咖啡酸、毛蕊花糖苷、柚皮苷、芦丁、阿魏酸、槲皮素)的HPLC-MS/MS分析方法。方法:采用Shim-pack VP-ODS色谱柱(2.0 mm×150 mm,5 μm),以甲醇-0.1%甲酸水为流动相,梯度洗脱,流速0.2 mL·min-1,柱温30℃,进样量5 μL;质谱采用ESI离子源,多反应监测(MRM)的负离子扫描方式进行检测。结果:绿原酸、咖啡酸、毛蕊花糖苷、柚皮苷、芦丁、阿魏酸、槲皮素的线性范围分别为50~4 000、100~4 000、50~4 000、50~4 000、50~4 000、200~4 000和50~4 000 ng·mL-1,平均回收率(n=6)分别为96.5%、93.3%、102.6%、97.8%、94.3%、91.5%和101.4%。精密度、重复性和稳定性良好;并符合方法学考察要求。丹桂中上述7个成分含量分别为(8.73±0.12)、(0.44±0.06)、(99.32±1.95)、(0.41±0.22)、(0.42±0.22)、(0.95±0.23)和(2.04±0.08)mg·g-1,金桂中上述7个成分含量分别为(6.68±0.08)、(0.64±0.02)、(98.67±1.50)、(0.14±0.05)、(1.56±0.08)、(3.88±0.50)和(2.89±0.00)mg·g-1,银桂中上述7个成分含量分别为(6.00±0.20)、(0.12±0.02)、(73.46±0.14)、(0.16±0.08)、(1.18±0.11)、(0.46±0.01)和(3.38±0.03)mg·g-1结论:所建立的方法可用于桂花中绿原酸、咖啡酸、毛蕊花糖苷、柚皮苷、芦丁、阿魏酸和槲皮素7个多酚类活性成分的同时测定。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish an HPLC-MS/MS method for the simultanneous determinnation of seven active ingredients (chlorogenic acid,caffeic acid,verbascoside,naringin,rutin,ferulic acid,and quercetin)in three kinds of Osmanthus fragrans (Dangui,Jingui and Yingui).Methods: The analysis was performed on a Shim-pack VP-ODS column (2.0 mm×150 mm,5 μm)using a mobile phase consisting of methanol and 0.1% formic acid at a flow rate of 0.2 mL·min-1 by gradient elution.The column temperature was set at 30℃,and the injection volume was 5 μL.The target compounds were analyzed by the negative ion multiple reaction monitoring (MRM)mode.Results: The linear ranges of chlorogenic acid,caffeic acid,verbascoside,naringin,rutin,ferulic acid and quercetin were 50-4 000,100-4 000,50-4 000,50-4 000,50-4 000,200-4 000 and 50-4 000 ng·mL-1,respectively.The average recoveries (n=6)were 96.5%,93.3%,102.6%,97.8%,94.3%,91.5% and 101.4%,respectively.Precision,repeatability and stability were in line with the methodological requirements.The contents of the above seven components in Dangui were (8.73±0.12), (0.44±0.06), (99.32±1.95), (0.41±0.22), (0.42±0.22), (0.95±0.23)and (2.04±0.08)mg·g-1,respectively.The contents of the above seven components in Jingui were (6.68±0.08), (0.64±0.02), (98.67±1.50), (0.14±0.05), (1.56±0.08), (3.88±0.50)and (2.89±0.00) mg·g-1;the contents of the above seven components in Yingui were (6.00±0.20), (0.12±0.02), (73.46±0.14), (0.16±0.08), (1.18±0.11), (0.46±0.01),and (3.38±0.03)mg·g-1.Conclusion: The established method can be used to determinate seven polyphenols of Osmanthus fragrans simultaneously.

-----参考文献:---------------------------------------------------------------------------------------

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