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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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超高效液相色谱法同时测定广藿香中6个成分的含量

Simultaneous determination of 6 components in Pogostemonis Herba by ultra performance liquid chromatography

作者(英文):
分类号:R917
出版年·卷·期(页码):2018,38 (8):1331-1336
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:建立同时测定广藿香中新西兰牡荆苷2、毛蕊花糖苷、异毛蕊花糖苷、列当苷、藿香黄酮醇和广藿香酮的超高效液相色谱(UPLC)分析方法。方法:采用Waters BEH C18(100 mm×2.1 mm,1.7 μm)色谱柱;流动相为乙腈-0.1%磷酸溶液,梯度洗脱;流速0.4 mL·min-1;柱温30℃;检测波长309 nm。结果:新西兰牡荆苷2、毛蕊花糖苷、异毛蕊花糖苷、列当苷、藿香黄酮醇、广藿香酮的线性范围分别为2.18~109.00、8.66~433.11、2.12~106.00、1.81~90.40、0.90~45.00、1.09~54.24 μg·mL-1r2>0.999 7);平均回收率(n=9)为99.1%~100.7%,精密度、重复性、稳定性均符合有关规定;10批样品中6个成分的含量范围分别为0.137~0.959、0.980~5.877、0.306~1.363、0.273~1.169、0.136~0.387、0.292~1.619 mg·g-1结论:本法经方法学验证,可用于广藿香质量控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective:To establish a UPLC method for simultaneous determination of vicenin-2,verbascoside,isoverbascoside,crenatoside,pachypodol and pogostone in Pogostemonis Herba. Methods:Analysis was performed on a Waters BEH C18 column(100 mm×2.1 mm,1.7μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid in gradient mode. The flow rate was 0.4 mL·min-1. The column temperature was maintained at 30℃ and the detection wavelength was set at 309 nm. Results:Vicenin-2,verbascoside,isoverbascoside,crenatoside,pachypodol and pogostone showed a good linear relationship within the ranges of 2.18-109.00 μg·mL-1,8.66-433.11 μg·mL-1,2.12-106.00 μg·mL-1,1.81-90.40 μg·mL-1,0.90-45.00 μg·mL-1 and 1.09-54.24 μg·mL-1,respectively(r2>0.999 7). The average recoveries of the six compounds were within 99.1%-100.7%. Results of precision,stability and reproducibility test were satisfactory. The contents of six components in ten batches of samples were 0.137-0.959 mg·g-1,0.980-5.877 mg·g-1,0.306-1.363 mg·g-1,0.273-1.169 mg·g-1,0.136-0.387 mg·g-1 and 0.292-1.619 mg·g-1,respectively. Conclusion:The established method can be used for the quality control of Pogostemonis Herba.

-----参考文献:---------------------------------------------------------------------------------------

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